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ARS Home » Midwest Area » Wooster, Ohio » Application Technology Research » Research » Publications at this Location » Publication #73882
Title: IDENTIFICATION OF BACTERIA WITH TWO COMMERCIAL SYSTEMS

Author
item Klein, Michael
item RICE, V - ENTOMOLOGY DEPT/OSU-OARDC

Submitted to: Entomological Society of America Miscellaneous Publications
Publication Type: Abstract Only
Publication Acceptance Date: 8/30/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Numerous bacterial isolates have been obtained from scarab larvae with signs and symptoms similar to amber disease in the New Zealand grass grub caused by Serratia spp. We have used two systems to identify our isolates obtained from Serratia selective media. The more basic system (Enterotube(R) II) was designed for clinical specimens and relies on 16 biochemical tests for differentiation of bacteria. The second system (MicrostationR) is more sophisticated and uses 95 biochemical tests and a computer aided identification procedure. Fifty five isolates were characterized as being in the genus Serratia using the Enterotubes. About half of the identifications were confirmed by the Microstation. Using the Microstation, eighteen isolates came out as different species of Serratia or different genera of bacteria than those identified by the Enterotubes. Given the increased number of biochemical tests and species in its data banks, the Microstation appears to be a more reliable indicator of bacterial identification for isolates from white grubs and soil.