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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #70150
Title: RAPD FINGERPRINTING AND PHYLOGENETIC ANALYSIS OF DIDYMELLA BRYONIAE AND PHOMA SPECIES

Author
item SOMAI, B - CLEMSON UNIVERSITY
item KEINATH, ANTHONY - CLEMSON UNIVERSITY
item ZITTER, THOMAS - CORNELL UNIVERSITY
item DEAN, RALPH - CLEMSON UNIVERSITY
item Farnham, Mark

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: D. bryoniae, the causal agent of gummy stem blight (GSB), and nonpathogenic Phoma spp. frequently are confused because both can be isolated simultaneously from cucurbits with GSB symptoms. The process of differentiating and identifying these fungi is difficult and time-consuming. Random amplified polymorphic DNA (RAPD) analysis with random oligonucleotide primers was used to "fingerprint" 61 isolates of these fungi from SC, NY, NC, FL, AZ and WI. Phylogenetic analysis using parsimony (PAUP) of RAPD data resulted in three clusters: D. bryoniae (35 isolates) and Phoma (14 isolates) clusters and an unidentified cluster (12 isolates). Pathogenicity tests conducted in a growth chamber indicated that only isolates of D. bryoniae caused disease on cantaloupe seedlings. Unique RAPD fragments are being cloned and will be sequenced to generate nested primers to enable PCR detection of the three clusters. PCR detection will provide a more rapid and reliable method of distinguishing among isolates in these three clusters and, therefore, will aid in accurate diagnosis.