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ARS Home » Midwest Area » Columbus, Ohio » Soil Drainage Research » Research » Publications at this Location » Publication #69580
Title: OPTIMIZATION OF SILVER-STAINING AFLP TECHNIQUE FOR SOYBEAN

Author
item Vantoai, Tara
item PENG, JIQING - THE OHIO STATE UNIVERSITY
item ST MARTIN, STEVE - THE OHIO STATE UNIVERSITY

Submitted to: Soybean Genetics Newsletter
Publication Type: Research Notes
Publication Acceptance Date: 4/5/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The AFLP technique is a very efficient DNA marker technique. Using a single primer pair, we were able to identify 14 polymorphic bands among two adapted and four plant introduction strains. Since Taq polymerase is expensive, the high number of polymorphism detected makes this technique more cost efficient than the RAPD technique. The technique is sensitive to the reproducibility of the thermoprofile within and across experiments. Silver-staining AFLP, while eliminating the use of radioactive isotopes, requires more skilled labor than the 32P or 33P-AFLP technique, and certainly more skilled labor than the RAPD technique.