Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/1995
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Bacterial, mycoplasmal, and viral infections have been shown to dramatically increase circulating TNF levels in swine. At low levels, TNF is beneficial and aids in host defense against these pathogens. However, excessive TNF production can lead to death in acute cases or cachexia with chronic TNF exposure. We are interested in reducing the activity of excess TNF in an effort to reduce the impact of Gram- negative bacterial infection in swine. Blockade of TNF by administration of recombinant soluble TNF receptors or INFR-Ig fusion proteins protects mice from the lethal effects of LPS and Gram- negative septicemia. Since TNF receptors bind to TNF in a species- specific fashion, we began to search for the porcine homolog of the human p55 TNF receptor (TNF-R1). We designed PCR primers corresponding to regions of homology between the human and mouse sequences. Using cDNA from a porcine TNF-responsive cell line, PK(15) (ATCC CCL33), as a template, we amplified a 475 bp fragment of the porcine TNF-R1 gene. Sequence analysis showed that this fragment has 78% homology with the human gene and 71% homology with the mouse gene in this region. This fragment codes for approximately 85% of the extracellular domain of porcine TNF-R.