Skip to main content
ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #411108
Research Project: Host-Pathogen Interactions Affecting Wheat and Barley

Location: Cereal Crops Improvement Research

Title: Biparental and natural population genetics identify Pyrenophora teres f. teres loci associated with a broadly effective barley resistance

Author
item SKIBA, RYAN - Oak Ridge Institute For Science And Education (ORISE)
item Wyatt, Nathan
item LI, JINLING - North Dakota State University
item KARIYAWASAM, GAYAN - North Dakota State University
item RICHARDS, JONATHON - Louisiana State University Agcenter
item EFFERTZ, KARL - Washington State University
item REHAM, SAJID - Olds College Centre For Innovation
item BRUEGGEMAN, ROBERT - Washington State University
item Friesen, Timothy

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/6/2023
Publication Date: 3/12/2024
Citation: Skiba, R., Wyatt, N.A., Li, J., Kariyawasam, G., Richards, J.K., Effertz, K., Reham, S., Brueggeman, R., Friesen, T.L. 2024. Biparental and natural population genetics identify Pyrenophora teres f. teres loci associated with a broadly effective barley resistance. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Pyrenophora teres f. teres, the fungal pathogen responsible for the foliar barley disease net form net blotch (NFNB), is an increasingly significant pathogen of barley worldwide. Though many genetic sources of resistance to NFNB have been identified, few are as broadly resistant as barley line CIho5791. To identify the genetic factors underlying virulence/avirulence on CIho5791, we created a biparental mapping population using a Moroccan P. teres f. teres isolate MorSM40-3 displaying substantial virulence crossed with the CIho5791-avirulent Canadian reference isolate 0-1. Whole-genome sequencing was performed for 103 MorSM40-3 × 0-1 progeny and used to create a saturated genetic map that was used to identify major quantitative trait loci (QTL) associated with virulence/avirulence on CIho5791. Major QTL were identified on chromosomes (Ch) 1 and 8 accounting for 27% and 15% of disease reaction type variation, respectively. While MorSM40-3 contributed the virulent allele at the Ch1 locus, the avirulent parent 0-1 contributed the virulent allele at the Ch8 locus, indicating an epistatic interaction between the two loci, as this Ch8 virulent allele appeared to be functional only in the presence of the MorSM40-3 Ch1 allele. We then screened a natural P. teres f. teres population on CIho5791 and used the resulting data to perform a genome wide association study (GWAS) using the natural population as well as a subset of this population representing only the Moroccan and North American isolates. The results of these analyses indicated a strong association with virulence/avirulence at the Ch1 and Ch8 loci we had previously identified using the biparental population. Additionally, long and short read sequencing were used to assemble and polish a reference quality genome for isolate MorSM40-3 and RNA-seq data from multiple infection timepoints was used to generate gene annotations indicating multiple candidate effector genes in the Ch1 and Ch8 loci.