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ARS Home » Pacific West Area » Riverside, California » National Clonal Germplasm Repository for Citrus » Research » Publications at this Location » Publication #409284

Research Project: Conservation, Management and Distribution of Citrus and Date Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository for Citrus

Title: Multiplex qPCR detection of ‘Candidatus Liberibacter spp.’ and Spiroplasma citri

item OSMAN, F - University Of California, Davis
item BODAGHI, S - University Of California, Riverside
item Krueger, Robert
item Yokomi, Raymond - Ray
item KUMAGAI, L - California Department Of Food And Agriculture
item VIDALAKIS, G - University Of California, Riverside

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Candidatus Liberibacter spp.’ and Spiroplasma citri (S. citri) are phloem-limited bacteria, associated with the citrus diseases Huanglongbing (HLB) and stubborn disease (CSD), respectively. CSD is endemic in California and has symptoms in citrus that can be easily confused with those of HLB (Fig. 1). Therefore, a real-time multiplex quantitative polymerase chain reaction (qPCR) assay for the simultaneous detection of all ‘Candidatus Liberibacter spp.’ (asiaticus (CLas), americanus (CLam), and africanus (CLaf) and S. citri was developed and validated using different fluorescently labeled minor groove binding qPCR probes. The capacity of the multiplex qPCR assay in detecting the pathogens was compared to singleplex qPCR assays designed specifically for each pathogen. The specificity and sensitivity of the updated qPCR assay was optimal and measuring the intra and inter assay variations confirmed the reproducibility and repeatability of the assay.