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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Healthy Body Weight Research » Research » Publications at this Location » Publication #407895

Research Project: Dietary and Physical Activity Guidance for Weight Loss and Maintenance

Location: Healthy Body Weight Research

Title: Chokeberry reduces inflammation in the buffy coat of american indians and in isolated human preadipocyte cells

item BRUNELLE, DALE - Oak Ridge Institute For Science And Education (ORISE)
item LARSON, KATE - Former ARS Employee
item Bundy, Amy
item Roemmich, James
item STEELE, JOEL - University Of North Dakota
item WARNE, DONALD - University Of North Dakota
item REDVERS, NICOLE - University Of North Dakota

Submitted to: Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/14/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Objectives: Chokeberry, Aronia melanocarpa, was consumed by American Indians as food and a means of preventing chronic diseases, but mechanisms of its health effects are unclear. The objective was to determine effects of Chokeberry juice extract (CBE) on inflammation measured by Interluekin-6 (IL-6) expression and epigenetic regulation of the promoter region of the IL-6 gene in the buffy coat from blood of American Indians (AI) and on isolated human preadipocyte cells (HPA) treated with CBE. Methods: A human study included 46 AI participants who consumed 100mg CBE twice per day for six weeks. The buffy coat was isolated from a blood draw before and after six weeks CBE consumption. The HPA cell culture study pretreated cells with 2µM CBE for 3h. Followed by 3h treatment of either: 0.17mM bovine serum albumin (BSA) as control; 0.1mM palmitic acid (PA); BSA with 2µM CBE; or PA with 2µM CBE. For both studies, IL-6 mRNA expression and DNA methylation was determined. HPA histone H3 acetylation was analyzed. Results: From ANOVA, consumption of CBE reduced (p=0.005) buffy coat IL-6 expression, and HPA treated with CBE decreased (p=.004) PA-induced IL-6 expression. Buffy coat DNA methylation was reduced at CpG site (-227) in AI (p=0.066), while HPA DNA methylation increased at CpG site (-118) in CBE group compared to control (p=.026) and CBE+PA (p=.042) groups. There was no difference in histone H3 acetylation. Conclusions: CBE-induced IL-6 promoter methylation exerts a protective effect against inflammation as measured by IL-6 mRNA expression via regulating IL-6 promoter region methylation.