Location: Northwest Irrigation and Soils ResearchTitle: Factors influencing agrobacterium mediated transient transformation of sugar beet
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/7/2023
Publication Date: N/A
Technical Abstract: While Agrobacterium mediated plant transformation has proven its worth to crop enhancement and breeding platforms, some crop species such as sugar beet are recalcitrant to agrobacterium mediated transformation or transformation events are so infrequent that it makes it cost/time prohibitive for most plant biology labs to pursue. Therefore, determining the factors influencing efficient agrobacterium transformation in sugar beet would prove useful in the establishment of sugar beet transformation pipelines. Factors influencing efficient agroinfiltration were investigated including different strains of Agrobacterium, media composition, bacteria/plant co-incubation, and plant growth conditions. A significant reduction in transgene silencing was observed allowing for high expression of transgene to be maintained for as long as 5 weeks after agroinfiltration. This was achieved by incorporating optimal promoter/5'-leader and terminator elements into the transgene T-DNA cassette. Overall, the results presented will aid in the establishment of a routine agroinfiltration-mediated transient method for sugar beet.