Location: Healthy Body Weight ResearchTitle: Chokeberry reduces inflammation in human preadipocytes
|BRUNELLE, DALE - Oak Ridge Institute For Science And Education (ORISE)|
|LARSON, KATE - Former ARS Employee|
|WARNE, DONALD - University Of North Dakota|
|REDVERS, NICOLE - Western University|
Submitted to: Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/18/2023
Publication Date: 5/18/2023
Citation: Brunelle, D.C., Larson, K., Bundy, A.N., Roemmich, J.N., Warne, D., Redvers, N. 2023. Chokeberry reduces inflammation in human preadipocytes. Symposium Proceedings. 1.
Technical Abstract: Objectives: Chokeberry, Aronia melanocarpa, used as a traditional food by American Indian tribes has cardioprotective, anti-diabetic, and anti-inflammatory properties. Palmitate (PA) induces inflammatory gene expression. The objective was to determine the effects chokeberry juice extract (CBE) and PA have on interleukin-6 (IL-6) gene expression, IL-6 DNA methylation and histone H3 acetylation in human pre-adipocyte cells (HPA). Methods: HPA were pretreated with CBE. Cells were then treated with bovine serum albumin (BSA) for a control; PA; BSA with CBE; or PA with CBE. RT-qPCR analysis of IL-6 mRNA expression was normalized to 18s rRNA expression. The IL-6 promoter region was tested for DNA methylation. Western blot determined histone H3 acetylation on lysine residues K9, K14, and K18. One-way ANOVAs determined significance. Results: HPA treated with PA increased (p<0.0001) IL-6 mRNA compared to control and CBE groups. Treatment of HPA with CBE decreased (p=.004) PA-induced IL-6 mRNA expression. IL-6 DNA methylation increased at CpG site (-118) in CBE group compared to control (p=.026) and CBE+PA (p=.042). No difference in histone H3 acetylation. Conclusions: CBE-induced IL-6 promoter methylation exerts a protective effect against PA-induced inflammation in HPA.