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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #402150

Research Project: Mapping Crop Genome Functions for Biology-Enabled Germplasm Improvement

Location: Plant, Soil and Nutrition Research

Title: Regulatory networks governing nitrogen use efficiency in maize and sorghum

item BRAYNEN, JANEEN - Cold Spring Harbor Laboratory
item ZHANG, LIFANG - Cold Spring Harbor Laboratory
item KUMARI, SUNITA - Cold Spring Harbor Laboratory
item KUMAR, VIVEK - Cold Spring Harbor Laboratory
item OLSON, ANDREW - Cold Spring Harbor Laboratory
item REGULSKI, MICHAEL - Cold Spring Harbor Laboratory
item LISERON-MONFILS, CHRISTOPHE - National Research Council - Canada
item GAUDINIER, ALLISON - University Of California Berkeley
item FRANK, MARY - Corteva Agriscience
item Ware, Doreen
item SHEN, BO - Corteva Agriscience

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/16/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Regulation of nitrogen availability is vital to increase crop production and reduce negative impacts on the environment. Previous studies of nitrogen availability under limiting conditions in Arabidopsis used an enhanced yeast-one hybrid assay (Y1H) gene regulatory network (GRN) to understand the response to nitrogen. However, the early response of nitrogen availability using a Y1H GRN network in crop species, such as maize is unknown. Here, we constructed a yeast one hybrid GRN for NUE in maize and used temporal expression patterns to profile the early response to nitrogen limitation and recovery. Key transcription factors (TFs) that regulate maize genes were involved in NUE with 1625 protein-DNA-interaction (PDI). Furthermore, 35% of the interactions in the network were conserved among various nitrogen-related processes between Arabidopsis and maize. Among such, three TF families were over-represented, including ERF, bZIP, and MYB. To assess if such regulated interaction is conserved in other crop species, we used the Gramene comparative database to obtain orthologs to project the NUE network from maize to sorghum. The NUE networks identified here can provide critical insight into the early response to nitrogen regulation for agronomically important crops. This work is funded by the US Department of Agriculture, Agriculture Research Service under Award Number 8062-21000-044-00D.