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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #400034

Research Project: Host-Pathogen Interactions Affecting Wheat and Barley

Location: Cereal Crops Improvement Research

Title: Host and pathogen genetics reveal an inverse gene-for-gene association in the P. teres f. maculata – barley pathosystem

item SKIBA, RYAN - Oak Ridge Institute For Science And Education (ORISE)
item Wyatt, Nathan
item KARIYAWASAM, GAYAN - North Dakota State University
item Yang, Shengming
item BRUEGGEMAN, ROBERT - Washington State University
item Friesen, Timothy

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/13/2023
Publication Date: 1/13/2023
Citation: Skiba, R., Wyatt, N.A., Kariyawasam, G., Yang, S., Brueggeman, R., Friesen, T.L. 2023. Host and pathogen genetics reveal an inverse gene-for-gene association in the P. teres f. maculata – barley pathosystem. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Spot-form net blotch (SFNB) of barley is a destructive barley leaf disease caused by the fungal pathogen Pyrenophora teres f. maculata. To better understand the barley-P. teres f. maculata interaction, we created a host population from a cross of the resistant barley line PI 67381 and the susceptible line Hockett. This population was screened with geographically diverse P. teres f. maculata isolates, and major quantitative trait loci (QTL) associated with resistance/susceptibility were identified on barley chromosomes 2H and 7H. Subsequently, we created a P. teres f. maculata pathogen population that was screened on PI 67381 and Hockett. QTL analyses identified major loci associated with virulence/avirulence on P. teres f. maculata chromosomes 1 and 2. The genetics of the SFNB interaction were further investigated by screening the PI 67381 × Hockett recombinant inbred population with progeny isolates that harbored the virulent allele at only one of the two identified loci. QTL analysis indicated that the Chr1 virulence was barley Chr2H and Chr2 virulence was targeting barley Chr7H. PI 67381 × Hockett F2 individuals were then screened with the single-virulence isolates to show that two dominant susceptibility genes in the host were facilitating the susceptible interaction. Lastly, previously generated gene annotation data was used to identify one and two predicted effector candidate genes underlying P. teres f. maculata virulence in Chr1 and Chr2, respectively. Collectively, this work shows that P. teres f. maculata is using multiple effectors to target dominant host susceptibility genes to colonize and complete its pathogenic life cycle.