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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #394165

Research Project: Improvement of Biotic Stress Resistance in Durum and Hard Red Spring Wheat Using Genetics and Genomics

Location: Cereal Crops Research

Title: Genetic mapping and characterization of Hessian fly resistance in barley

item KARKI, MANILA - North Dakota State University
item LIU, ZHAOHUI - North Dakota State University
item Yang, Shengming

Submitted to: American Pathological Society North Central Division Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2022
Publication Date: 6/21/2022
Citation: Karki, M., Liu, Z., Yang, S. 2022. Genetic mapping and characterization of Hessian fly resistance in barley. American Pathological Society North Central Division Annual Meeting 2022. Page 32.

Interpretive Summary:

Technical Abstract: Hessian fly (Mayetiola destructor) is one of the most important species of fly causing significant yield losses in cereals including wheat, barley, and rye. Interestingly, the outcome of interaction between Hessian fly and its host is also controlled by the gene-for-gene model. Virulent larvae use the salivary effector to suppress host defence responses, which leads to a compatible interaction. However, recognition of the larval salivary effector by the cognate R protein activates defence reactions, restricting colonization of Hessian fly on the host. It is well known that genetic resistance is the most efficient and cost-effective strategy for pathogen control. Hessian fly resistance has been extensively searched in wheat, and at least 37 resistance loci (H1-H37) have been reported. Although Hessian fly is a significant pest of barley, identification of genetic resistance in barley has never been conducted. In the present study, genome-wide association mapping was performed to identify barley resistance to Hessian fly using a core collection of 240 lines. Of those, 210 lines were susceptible, indicating an urgent need to enhance barley resistance to Hessian fly. A total of 5 resistance loci was identified on 1H, 3H, 4H, 5H, and 6H, respectively. The locus on 4H (named HvRHF1 hereafter) showed a significant effect on disease phenotype, and it was selected for fine mapping using a biparental population. Assisted with a total of 250 F2s, the HvRHF1 gene was delimited to a 1.5 Mb region containing at least four NBS-LRR genes and four protein kinase genes. Therefore, our study provided resistance resource for breeding programs, and it also offered a genetic map paving the foundation for cloning of the HvRHF1 gene.