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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #393914
Research Project: Molecular Genetic Tools Advancing the Application of Biotechnology for Crop Improvement

Location: Crop Improvement and Genetics Research

Title: Evaluation of novel surfactants for plant transformation

Author
item Huynh, Jennie
item HOTTON, SARA - Former ARS Employee
item Chan, Ronald
item SYED, YASRA - Former ARS Employee
item Thomson, James - Jim

Submitted to: BMC Research Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/23/2022
Publication Date: 12/8/2022
Citation: Huynh, J.N., Hotton, S.K., Chan, R., Syed, Y., Thomson, J.G. 2022. Evaluation of novel surfactants for plant transformation. BMC Research Notes. 15. Article 360. https://doi.org/10.1186/s13104-022-06251-5.
DOI: https://doi.org/10.1186/s13104-022-06251-5

Interpretive Summary: This work reports testing of novel surfactant compounds on the effect of Arabidopsis transformation. Three promoters were isolated from citrus and one from plum. Results demonstrate the surfactants BREAK THRU® S 200, BREAK THRU® S 240 and BREAK THRU® S 279 are superior to Silwet® L-77 for rates of Arabidopsis transformation.

Technical Abstract: The comparison of seven novel surfactants to the typically used Silwet® L-77 for floral dip Arabidopsis transformation and determination of effects on efficiency.Floral dip transformation has been consistently used for the past twenty years with little change in the original protocol. Here we explore the use of seven novel surfactants (BREAK THRU® OE 446, BREAK THRU® S 200, BREAK THRU® S 233, BREAK THRU® S 240, BREAK THRU® S 279, BREAK THRU® S 301 and BREAK THRU® SP 133) while in head-to-head comparison with Silwet® L-77 for effects on Arabidopsis transformation efficiency. Results indicate differences in relative transformation efficiencies ranging from -44% to +45% when using the various surfactants compared to Silwet® L-77. Surfactants BREAK THRU® S 200, BREAK THRU® S 240 and BREAK THRU® S 279 demonstrated the greatest enhancement in transformation.