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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #393568

Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Identification of genetic sources of resistance to the emerging Cucumber green mottle mosaic virus in cucumber

item ZIA, BAZGHA - Clemson University
item Weng, Yiqun
item CUTULLE, MATTHEW - Clemson University
item Ling, Kai-Shu

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2022
Publication Date: 11/1/2022
Citation: Zia, B., Weng, Y., Cutulle, M., Ling, K. 2022. Identification of genetic sources of resistance to the emerging Cucumber green mottle mosaic virus in cucumber. American Phytopathological Society Annual Meeting. S3.167

Interpretive Summary: n/a

Technical Abstract: Cucumber green mottle mosaic virus (CGMMV) is a tobamovirus that has emerged in recent years to cause serious disease outbreaks on cucurbit crops around the world, including the U.S. With increasing efforts in off-shore commercial hybrid seed production and increasing global seed trade activities, this seed-borne virus and mechanically transmitted virus could initiate a new disease infection to seedlings from CGMMV-contaminated seeds. CGMMV can cause severe disease symptoms including leaf mottling and fruit decay on infected cucurbits. Breeding for resistance is the most effective means in viral disease management. In the present study, we focused our efforts in studying CGMMV infection and identification of resistance sources from selected cucumber breeding lines. Using mechanical transmission, a total of 50 elite cucumber breeding lines were tested for resistance to CGMMV. Disease symptoms were scored on a rating of 0-4, with 0 being healthy with no symptom and 4 being severe with mosaic and mottling. Serological tests (enzyme-linked immunosorbent assay) were used to assess the virus infection on test plants. As a result, three lines were considered tolerant to CGMMV as they developed mild to no visible symptom, although plants were still infected. The F1 and F2 populations developed will be used to assess for disease incidence and genetic inheritance of resistance. Studies are underway to conduct single nucleotide polymorphism profiling for the developed population. Genetic components of resistance to CGMMV and marker-assisted selection will be discussed.