Complete genome sequence resource for pseudomonas amygdali pv. loropetali strain AAC causing bacterial gall of loropetalum chinense

Authors: JIA, JIAYUAN - Mississippi State University; Copes, Warren; PHILLIPS, KATE - Mississippi State University; LU, SHI-EN - Mississippi State University

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/25/2022
Publication Date: 9/1/2022
Citation: Jia, J., Copes, W.E., Phillips, K., Lu, S. 2022. Complete genome sequence resource for pseudomonas amygdali pv. loropetali strain AAC causing bacterial gall of loropetalum chinense. Plant Disease. 106(9):0191-2917. https://doi.org/10.1094/PDIS-04-22-0919-A.
DOI: https://doi.org/10.1094/PDIS-04-22-0919-A

Interpretive Summary: Bacterial knot disease was first reported on Loropetalum cultivars in the US in 2013. Loropetalum is a popular landscape shrub in the southeastern US. Bacterial knot, caused by Pseudomonas amygdali pv. loropetali, has become the most serious disease impacting commercial production of Loropetalum in plant nurseries, yet little is known about the pathogen and how to optimize control. Furthermore, Pseudomonas amygdali falls within a large plant pathogenic Pseudomonas syringae species complex (Pssc) where little is known about many of the bacteria in this species complex. We report the first whole genome sequence of P. amygdali pv. loropetali, provide a breakdown of the number genes within the genome, identify the types and functions of some of the genes and compare the genome against other key bacteria within the Pssc. The genome will provide a valuable resource for researchers studying pathogen virulence and plant host resistance, developing primers used for identification of the pathogen and evaluating chemical resistance in field populations of the pathogen.

Technical Abstract: Pseudomonas amygdali pv. loropetali is the casual agent of bacterial gall disease on Loropetalum. Here, we report the complete genome of P. amygdali pv. loropetali AAC, which represents the first whole genome sequence of the pathovar loropetali. Strain AAC was found to have one circular chromosome 6,241,584 bp in size with 58.20 % G+C content and was predicted to harbor 5,279 protein coding genes in the putative 5,647 genes. In total, 16 rRNAs, 66 tRNAs, and 4 ncRNAs were identified, and 282 pseudogenes were annotated as well. This complete genome will provide a valuable resource for further studies on understanding Loropetalum-P. amygdali pv. loropetali interactions.