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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Production and Genetic Improvement Research Unit » Research » Publications at this Location » Publication #392948

Research Project: Genetic Improvement of Blackberry, Red and Black Raspberry, Blueberry, and Strawberry

Location: Horticultural Crops Production and Genetic Improvement Research Unit

Title: Complete genome sequence of a moroccan isolate of cereal chlorotic mottle virus

item ALVAREZ-QUINTO, ROBERT - University Of Minnesota
item LOCKHART, BENHAM - University Of Minnesota
item SERRANO, JOANA - University Of Minnesota
item Grinstead, Sam
item Mollov, Dimitre

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/24/2022
Publication Date: 7/14/2022
Citation: Alvarez-Quinto, R., Lockhart, B., Serrano, J., Grinstead, S.C., Mollov, D.S. 2022. Complete genome sequence of a moroccan isolate of cereal chlorotic mottle virus. Archives of Virology.

Interpretive Summary: In this research we describe the complete genomic sequence of cereal chlorotic mottle virus (CCMoV). This virus was described originally in 1979 and is known to infect cereal crops (including barley, oat, and wheat) and weeds from the grass family. With this new data the virus can be properly classified and the genomic sequence used to develop detection protocols. This information will be useful to monitor virus distribution and aid in disease management.

Technical Abstract: Cereal chlorotic mottle virus (CCMoV) is a cicadellid-transmitted plant rhabdovirus associated with chlorotic and necrotic streaks on several gramineous hosts and weeds. The virus was initially described in 1979 in Australia but its genome has never been sequenced. In this study, the complete genome of a Moroccan isolate of CCMoV was generated by high throughput sequencing from infected oat leaves (Avena sativa). The genome is 13,800 nt long containing seven open reading frames (ORFs) arranged in the rhabdovirus canonical organization: 3’-nucleocapsid (N), phosphoprotein (P), unknown protein (p3), unknown protein (p4), matrix (M), glycoprotein (G), and the viral polymerase (L)-5’. Pairwise analysis showed that maize fine streak virus (MFSV, genus Gammanucleorhabdovirus) was the closest relative. The amino acid identity between homolog proteins from CCMoV and MFSV are as follows 59.27% (N), 36.7% (P), 24% (P3), 62% (P4), 43.70% (M), 49.15% (G), 60.93% (L). Based on the phylogenetic estimations and analog genome architecture CCMoV should be assigned as member of the genus Gammanucleorhabdovirus. The low identity observed between CCMoV and MFSV suggest that CCMoV is a distinct species within the Gammanucleorhabdovirus.