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ARS Home » Southeast Area » Auburn, Alabama » Soil Dynamics Research » Research » Publications at this Location » Publication #392739

Research Project: Conservation Systems to Improve Production Efficiency, Reduce Risk, and Promote Sustainability

Location: Soil Dynamics Research

Title: Molecular epidemiology of cotton leafroll dwarf virus in southeastern United States

item ESCALANTE, CESAR - Auburn University
item ZACCARON, MARCIO - Auburn University
item JACOBSON, ALANA - Auburn University
item BOWEN, KIRA - Auburn University
item STRAYER-SCHERER, AMANDA - Auburn University
item HAGAN, AUSTIN - Auburn University
item BROWN, STEVE - Auburn University
item GREENE, JEREMY - Clemson University
item MITTAPALLI, OMPRAKASH - Auburn University
item Balkcom, Kipling
item HUSETH, ANDERS - North Carolina State University
item MULVANEY, MIKE - Mississippi State University
item SMALL, IAN - University Of Florida
item KEMERAIT, BOB - University Of Georgia
item KELLY, HEATHER - University Of Tennessee
item ALLEN, TOM - Mississippi State University
item PRICE, TREY - Louisiana State University
item KERNS, DAVID - Texas A&M University
item FASKE, TRAVIS - University Of Arkansas
item KOEBERNICK, JENNY - Auburn University

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/11/2022
Publication Date: 12/26/2022
Citation: Escalante, C., Zaccaron, M., Jacobson, A., Bowen, K., Strayer-Scherer, A., Hagan, A., Brown, S., Greene, J.K., Mittapalli, O., Balkcom, K.S., Huseth, A., Mulvaney, M., Small, I., Kemerait, B., Kelly, H., Allen, T., Price, T., Kerns, D., Faske, T., Koebernick, J. 2022. Molecular epidemiology of cotton leafroll dwarf virus in southeastern United States [abstract]. Phytopathology. 112:S3.19. Plant Health 2022, Pittsburgh, PA, 08/6-10/2022.

Interpretive Summary:

Technical Abstract: Cotton leafroll dwarf virus (CLRDV) belongs to the genus Polerovirus in the family Solemoviridae and is transmitted by the cotton aphid (Aphis gossypii). Currently, CLRDV is considered an emerging disease with potential to cause yield loss in the cotton industry in the United States. The objective of this study was to characterize the distribution, incidence, symptomatology, and diversity of CLRDV in cotton aphids and cotton plants in the southeastern United States. Incidence and symptomatology of CLRDV in cotton was evaluated in sentinel plots during the 2019-2021 cotton growing seasons in 11 states. A total of eight cultivars were tested in a randomized complete block design with four replications. Total nucleic acids were extracted from cotton aphids and composite cotton leaf samples collected from the sentinel plots to determine presence of CLRDV by RT-PCR and to assess viral diversity via metagenomics analysis. CLRDV was detected in all plots and cultivars tested. Symptoms consisted of leaf rugosity, leaf drooping, bronzing of upper-canopy leaves, and increased vegetative growth. Symptom appearance was greatest in the coastal states. The number of positive samples tested by RT-PCR did not correlate with symptom appearance, therefore, symptomology could not be reliably used to determine virus presence. Results on viral genetic diversity collected from vector and host plants will be presented. Data generated in this study will support development of a holistic approach to sustainable management programs for mitigating cotton yield loss by CLRDV.