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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #391863
Research Project: Gene Discovery and Designing Soybeans for Food, Feed, and Industrial Applications

Location: Plant Genetics Research

Title: Breeding of ‘DND358’: a new soybean cultivar for processing soy protein isolate with a hypocholesterolemic effect similar to that of fenofibrate

Author
item SONG, BO - Northeast Agricultural University, China
item QIU, ZHENDONG - Northeast Agricultural University, China
item LI, MINGXUE - Northeast Agricultural University, China
item LUO, TINGTING - Northeast Agricultural University, China
item WU, QI - Heilongjiang Academy Of Agricultural Sciences
item Krishnan, Hari
item WU, JUNJIANG - Heilongjiang Academy Of Agricultural Sciences
item XU, PENGFEI - Northeast Agricultural University, China
item ZHANG, SHUZHEN - Northeast Agricultural University, China
item LIU, SHANSHAN - Northeast Agricultural University, China

Submitted to: Journal of Functional Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/2022
Publication Date: 3/5/2022
Publication URL: https://handle.nal.usda.gov/10113/7664820
Citation: Song, B., Qiu, Z., Li, M., Luo, T., Wu, Q., Krishnan, H.B., Wu, J., Xu, P., Zhang, S., Liu, S. 2022. Breeding of ‘DND358’: a new soybean cultivar for processing soy protein isolate with a hypocholesterolemic effect similar to that of fenofibrate. Journal of Functional Foods. 90. Article 104979. https://doi.org/10.1016/j.jff.2022.104979.
DOI: https://doi.org/10.1016/j.jff.2022.104979

Interpretive Summary: Soybeans contain several phytochemicals that promote human health, in part, by helping to reduce cholesterol levels. Several soy protein components are crucial for the reduced cholesterol effect, however, the individual role of various protein subunits in influencing the reduced cholesterol effects of soy protein were not previously explored. In the present study, we developed a new soy cultivar ‘DND358’ with an increase in the a subunit and arginine content. We produced a beverage powder from defatted ‘DND358’ soy flour and demonstrated that this beverage powder was as effective as fenofibrate, an alternative to statins for lowing blood cholesterol levels. Our study demonstrates positive benefits of soy protein isolates for human health. Further development of soybean cultivars like DND358 should benefit US farmers by promoting its wider use in health and food industry.

Technical Abstract: Soy proteins have hypocholesterolemic effects (HCE). Here, we developed a soybean cultivar ‘DND358’, which is superior for processing hypocholesterolemic soy protein isolate beverage powder (DND358-SPI). ‘DND358’ carries four recessive null alleles for the a subunit of 7S globulin and the G1, G2, and G4 subunits of 11S globulin (tetra null), was selected from a population derived from a three-way cross. The tetra null mutation resulted in a compensatory increase in the a' subunit level and arginine content. The oral administration (300 mg/kg/day) of DND358-SPI processed from ‘DND358’ significantly decreased the fatty liver symptoms and hepatic lipid accumulation; reduced the hepatic total cholesterol (TC), triacylglycerol (TG) levels and atherogenic index (AI) value, in rats fed a high-cholesterol diet. Conversely, DND358-SPI administration markedly elevated high-density lipoprotein cholesterol levels, to values significantly higher to those fed on fenofibrate (FF, 30 mg/kg/day), a hypocholesterolemic drug. The HCE of DND358-SPI were similar to that of FF.