|DOSKI, SHADIN - Former ARS Employee|
|JUSZCZAK, SAMANTHA - Agdia|
|GROTH-HELMS, DEBORAH - Agdia|
Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2022
Publication Date: 7/7/2022
Citation: Doski, S., Bolus, S.J., Grinstead, S.C., Juszczak, S., Groth-Helms, D., Mollov, D.S. 2022. Complete sequence and genome characterization of a new potexvirus isolated from chaenostoma cordatum. Archives of Virology. https://doi.org/10.1007/s00705-022-05520-9.
Interpretive Summary: Chaenostoma cordatum, known also as bacopa is an annual ornamental plant. Plant diseases including those caused by viruses reduce the ornamental plant value. Up to now it was known that bacopa can be infected by three viruses. In this study we investigated symptomatic bacopa plants. Our results revealed a new virus infecting bacopa. The complete genomic sequence of the virus was determined. These findings will facilitate development of diagnostic protocols and will help commercial growers, plant health officials, and consumers employ better control strategies for managing this new viral disease.
Technical Abstract: Leaves from the ornamental plant Chaenostoma cordatum (Thunb.) Benth. expressing virus-like symptoms were collected for pathogen testing, RNA extraction, and downstream analysis. Applying high throughput sequencing, we identified a virus with features consistent with those from the genus Potexvirus. We confirmed and completed the genome sequence using RT-PCR, cloning, Rapid Amplification of cDNA ends kits, and Sanger sequencing, revealing a complete viral genome of 6,071 nucleotides excluding the poly-A tail. Phylogenetic analysis of the RNA-dependent RNA polymerase sequence from the viral genome indicated that its closest relative is Plantago asiatica mosaic virus. Further analysis of the nucleotide and amino acid sequences revealed that it had diverged enough from other potexviruses to be considered a member of a new species.