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ARS Home » Northeast Area » Geneva, New York » Plant Genetic Resources Unit (PGRU) » Research » Publications at this Location » Publication #390757

Research Project: Management and Development of Apple, Cold-Hardy Grape, and Tart Cherry Genetic Resources and Associated Information

Location: Plant Genetic Resources Unit (PGRU)

Title: Tannins phenotyping in the Vitaceae reveals constellations of compositions intimately linked to genera and species

Author
item BRILLOUET, JEAN-MARC - Inrae
item ROMIEU, CHARLES - Inrae
item BACILIERI, ROBERTO - Inrae
item NICK, PETER - Karlsruhe Institute Of Technology
item TRIAS-BLASI, ANNA - Royal Botanic Gardens, Kew
item MAUL, ERIKA - Julius Kuhn Institute
item SOLYMOSI, KATALYN - Eotvos Lorand University
item SZELAK, PETER - University Of Pecs
item JIANG, JIAN-FU - Chinese Academy Of Agricultural Sciences
item SUN, LEI - Chinese Academy Of Agricultural Sciences
item ORTOLANI, DANIELLE - Jardin Exotique De Monaco
item Londo, Jason
item Gutierrez, Benjamin
item Prins, Bernard - Bernie
item REYNDERS, MARC - Botanic Garden Meise
item VANCAEKENBERGHE, FRANK - Botanic Garden Meise
item MAGHRADZE, DAVID - National Wine Agency Of Georgia
item MARCHAL, CECILE - Inrae
item SULTAN, AMIR - National Herbarium Program Of Pakistan Agricultural Research Council
item THOMAS, JEAN-FRANCOIS - Jardin Botanique De Lyon
item SCHERBERICH, DANIEL - Jardin Botanique De Lyon
item FULCRAND, HELENE - Inrae
item ROUMEAS, LAURENT - Inrae
item BILLERACH, GUILLAUME - Inrae
item SALIMOV, VUGAR - Azerbaijani Scientific Research Institute Of Viticulture And Winemaking
item MUSAYEV, MIRZA - Azerbaijan Academy Of Sciences
item UL ISLAM DAR, HUHAMMAD EJAZ - University Of Azad Jammu & Kashmir
item PELTIER, JEAN-BENOIT - Inrae
item GAUDEUL, MYRIAM - Herbarium Of The Muséum National D’Histoire Naturelle De Paris
item GRISONI, MICHEL - Cirad, France

Submitted to: Annals of Botany
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/4/2021
Publication Date: 6/14/2022
Citation: Brillouet, J., Romieu, C., Bacilieri, R., Nick, P., Trias-Blasi, A., Maul, E., Solymosi, K., Szelak, P., Jiang, J., Sun, L., Ortolani, D., Londo, J.P., Gutierrez, B.L., Prins, B.H., Reynders, M., Vancaekenberghe, F., Maghradze, D., Marchal, C., Sultan, A., Thomas, J., Scherberich, D., Fulcrand, H., Roumeas, L., Billerach, G., Salimov, V., Musayev, M., Ul Islam Dar, H., Peltier, J., Gaudeul, M., Grisoni, M. 2022. Tannins phenotyping in the Vitaceae reveals constellations of compositions intimately linked to genera and species. Annals Of Botany. https://doi.org/10.1093/aob/mcac077.
DOI: https://doi.org/10.1093/aob/mcac077

Interpretive Summary: Background and Aims: Condensed tannins, responsible for berry and wine astringency, may have been selected during grape domestication (Narduzzi et al., 2015). This work addresses the genetic diversity of condensed tannins throughout the Vitaceae phylogenetic tree. Methods Green berries and mature leaves of representative true-to-type members of the Vitaceae were collected before “véraison”, freeze-dried, pulverized, and condensed tannins were depolymerized by nucleophilic addition of 2-mercaptoethanol on C4 of the flavan-3-ol units in an organic acidic medium. Reaction products were separated and quantitated by UPLC/DAD/MS. Key Results and Conclusions The ability to include epigallocatechin (EGC) in condensed tannins disappeared independently, on both continental branches of the Vitaceae, with exceptional reversions to the ancestral EGC phenotype. This is particularly true in the Vitis genus, where we now find two radically distinct groups, the presence of EGC being strongly associated with the size of leaf margin teeth. Most Vitis species from Asia are devoid of EGC, while EGC is present in 50% of New World ones. Noticeably, the rare Asian EGC forming species are phylogenetically close to Vitis vinifera, the unique Vitis representative in Eurasia, forming EGC both in its wild and cultivated compartments. Consistent differences were also observed in the respective activities of the EGC and the galloylation biosynthesis pathways, that compete for photoassimilates and reductive power.

Technical Abstract: Background and Aims: Condensed tannins, responsible for berry and wine astringency, may have been selected during grape domestication (Narduzzi et al., 2015). This work addresses the genetic diversity of condensed tannins throughout the Vitaceae phylogenetic tree. Methods Green berries and mature leaves of representative true-to-type members of the Vitaceae were collected before “véraison”, freeze-dried, pulverized, and condensed tannins were depolymerized by nucleophilic addition of 2-mercaptoethanol on C4 of the flavan-3-ol units in an organic acidic medium. Reaction products were separated and quantitated by UPLC/DAD/MS. Key Results and Conclusions The ability to include epigallocatechin (EGC) in condensed tannins disappeared independently, on both continental branches of the Vitaceae, with exceptional reversions to the ancestral EGC phenotype. This is particularly true in the Vitis genus, where we now find two radically distinct groups, the presence of EGC being strongly associated with the size of leaf margin teeth. Most Vitis species from Asia are devoid of EGC, while EGC is present in 50% of New World ones. Noticeably, the rare Asian EGC forming species are phylogenetically close to Vitis vinifera, the unique Vitis representative in Eurasia, forming EGC both in its wild and cultivated compartments. Consistent differences were also observed in the respective activities of the EGC and the galloylation biosynthesis pathways, that compete for photoassimilates and reductive power.