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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #390531

Research Project: Genetic Improvement and Sustainable Production Systems for Sub-tropical and Tropical Crops in the Pacific Basin

Location: Tropical Plant Genetic Resources and Disease Research

Title: Complete genome organization and characterization of Hippeastrum latent virus

item XANG, XUPENG - University Of Hawaii
item LARREA-SARMIENTO, ANDRIANA - University Of Hawaii
item OLMEDO-VELARDE, ALEJANDRO - University Of Hawaii
item BORTH, WAYNE - University Of Hawaii
item Suzuki, Jon
item Wall, Marisa
item MELZER, MICHAEL - University Of Hawaii
item HU, JOHN - University Of Hawaii

Submitted to: Virus Genes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/1/2022
Publication Date: 4/15/2022
Citation: Xang, X., Larrea-Sarmiento, A., Olmedo-Velarde, A., Borth, W., Suzuki, J.Y., Wall, M.M., Melzer, M., Hu, J. 2022. Complete genome organization and characterization of Hippeastrum latent virus. Virus Genes.

Interpretive Summary: High throughput sequencing was used to determine the possible presence of plant viruses correlated with ringspot and mosaic symptoms on leaves of spider lily, a commonly grown ornamental in Hawaii. Results identify two closely related, but distinct plant carlaviruses from these diseased plants including the first characterization of the entire Hippeastrum latent virus (HiLV) genome. This is the first report of plant HiLV in spider lily and in the U.S.

Technical Abstract: The complete genome sequences of two carlaviruses were determined by high throughput sequencing of RNA extracted from ringspot and mosaic, disease symptom leaves of spider lily plants (Crinum asiaticum, family Amaryllidaceae) growing as landscape plants in Hawaii. One, named Nerine latent virus (NeLV) Hawaii with a genome of 8,281 nucleotide exhibited highest nucleotide identity and amino acid similarity of 95.5% and 96.0%, respectively to the genome sequence of an isolate of NeLV isolated from Narcissus sp. in Australia (JQ395044), whereas, the second, named Hippeastrum latent virus (HiLV)-Hawaii with a genome of 8,497 nucleotides exhibited the highest nucleotide identity and amino acid similarity, 84.3% and 88.7%, respectively to a sequence of previously uncharacterized HiLV isolate from the potted flowering plant, Amaryllis (Hippeastrum hybridum Hort) in Taiwan (DQ098905). The amino acid sequence similarities of replicase (Rep) and coat protein (CP) between HiLV-Hawaii and NeLV-Hawaii were 44.8% and 38.4%, respectively. Results of viral protein Rep and CP amino acid sequence comparisons from various carlaviruses provide evidence that HiLV and NeLV, previously classified as synonymous viruses are in fact, unique viruses. This is first report for the complete sequence, organization, and phylogenetic characterization of HiLV and the first report of HiLV in C. asiaticum in the USA.