Submitted to: Cryobiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/2/2022
Publication Date: 3/4/2022
Citation: Jenderek, M.M., Yeater, K.M., Ambruzs, B.D., Magby, J.T. 2022. Pretreatment Prunus avium (L) L. dormant buds increased viability after cryogenic storage. Cryobiology. 106:164-166. https://doi.org/10.1016/j.cryobiol.2022.03.001.
Interpretive Summary: Due to a lack of an effective ex situ preservation method, several genotypes of sweet cherry (P. avium) in the national collection were permanently lost, leaving only a small percentage of alive varieties. Cooperative research of ARS and Washington State University scientists identified compounds that enhanced viability of dormant winter buds to an acceptable level after storage in liquid nitrogen vapor. This finding provided a viable option to preserve sweet cherry in a long-term storge and protecting the collection from further losses caused by biotic and abiotic stressors.
Technical Abstract: Certain woody plant species may be cryopreserved by dormant winter buds (DBs) if their viability after liquid nitrogen vapor (LNV) exposure is >40% (viability standard for cryogenic storage). In our experience, sweet cherry (P. avium) DBs did not meet this standard. The objective of the study was to test if a pretreatment with cryoprotectants with addition of antioxidants, may increase viability to the standard. Dormant bud segments of four sweet cherry varieties ('Attika', 'Bing', 'Sandra Rose', and 'Skeena') were pretreated with ten different combinations of sucrose sucralose, and trehalose with or without antioxidants, and without a pretreatment, stored in LNV and tested for viability after. The highest viability was observed for DBs pretreated with 0.50 M trehalose (74.8+9.0% viability) and the lowest for DBs pretreated with sucralose (5.1+3.9%). The increased viability, due to the pretreatments, provided a viable option to preserve P. avium DBs in a long-term storage.