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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #389664

Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

Location: Peanut and Small Grains Research Unit

Title: Identification and characterization of the sorghum JAZ gene and their roles in plant defense

item SHRESTHA, KUMAR - Oklahoma State University
item Huang, Yinghua

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/12/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Identification of Jasmonate ZIM-domain (JAZ) genes and elucidation of their roles in the jasmonic acid (JA) signaling pathway are important as they are link several molecular mechanisms in plant growth and development as well as plant defense. Here we report the results from our recent studies on the identification of sorghum JAZ genes and analysis of their expression in sorghum seedlings exposed to several phytohormones and pest aphid. Eighteen JAZ genes (SbJAZ) were identified in sorghum through genome-wide analysis. Based on the analysis of their expression products, all SbJAZ proteins possess the conserved TIFY and Jas domains but they formed a phylogenetic tree having five clusters with the orthologs of other plant species. The evolutionary analysis indicated the duplication event was a major force for an expansion of the SbJAZ gene family. In silico analysis of the SbJAZ gene promoters indicates that SbJAZ5, SbJAZ6, SbJAZ13, SbJAZ16 and SbJAZ17 are rich in stress-related cis-elements. In addition, expression profiling of the SbJAZ genes showed differential expression in response to phytohormones treatment (JA, ET, ABA, GA) and sugarcane aphid (SCA) between two recombinant inbred lines (RILs), resistant (RIL521) and susceptible (RIL609) to SCA. Notably, upregulation of SbJAZ1, SbJAZ5, SbJAZ13 and SbJAZ16 in resistant RIL during JA treatment and SCA infestation suggests putative functions in stress-related defense and to balance the plant defense to promote growth. In summary, those data can not only provide valuable information about sorghum JAZ gene family but also facilitate characterization of the function of SbJAZ genes in sorghum.