|ALVAREZ-QUINTO, ROBERT - University Of Minnesota|
|SERRANO, JOANA - University Of Minnesota|
|OLSZEWSKI, NEIL - University Of Minnesota|
|LOCKHART, BEN - University Of Minnesota|
Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/7/2021
Publication Date: 1/13/2022
Citation: Alvarez-Quinto, R., Serrano, J., Olszewski, N., Grinstead, S.C., Mollov, D.S., Lockhart, B. 2022. Complete genome sequence of two isolates of spiraea yellow leafspot virus (genus: badnavirus) from spiraea x bumalda 'Anthony Waterer'. Archives of Virology. https://doi.org/10.1007/s00705-021-05336-z.
Interpretive Summary: pirea is an ornamental crop (family Rosaceae) widely used in landscape settings. In this study, we used high throughput sequencing technology (HTS) to assess potential virus infection. HTS analysis revealed infection by a virus related to badnaviruses. The complete genomic sequence from two isolates, one from Minnesota and one from Maryland were completed. These findings will facilitate reliable detection methods and aid in nursery production of virus free material.
Technical Abstract: Two isolates of Spiraea yellow leafspot virus (SYLSV ) were completely sequenced from Spiraea (Spiraea x bumalda) ‘Anthony Waterer’. Samples were collected in Minnesota and Maryland between 2018-2020 from plants showing virus-like symptoms including yellow spotting and leaf deformation. The complete genome of SYLSV was 8,017bp in length for both isolates. SYLSV-MN (Minnesota) and SYLSV-MD (Maryland) share 95% of identity at the nucleotide level. Both isolates have the same genome organization containing three open reading frames (ORFs), with ORF3 being the largest encoding the putative polyprotein of 232 kDa with conserved domains including Zinc finger, pepsin-like aspartate protease, reverse transcriptase (RT), and RNase H. Pairwise comparisons between members of the genus badnavirus showed that Gooseberry vein banding associated virus GB1 (HQ852248) and Rubus yellow net virus isolate Baumforth's Seedling A (KM078034) were the closest related virus sequences to SYLSV sharing 73% of identity at the nucleotide level. Bacilliform virions of 150 nm x 30 nm were the only particles observed in symptomatic plants but not in asymptomatic samples.