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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #385253

Research Project: Management Strategies for Invasive Aphid Pests of Cereals

Location: Peanut and Small Grains Research Unit

Title: The recent evolutionary rescue of a staple crop depended on over half a century of global germplasm exchange

item MULETA, KEBEDE - Kansas State University
item FELDERHOFF, TERRY - Kansas State University
item WINANS, NOAH - Kansas State University
item WALSTEAD, RACHEL - Hudsonalpha Institute For Biotechnology
item CHARLES, JEAN RIGAUD - Quisqueya University
item Armstrong, John
item MAMIDI, SUJAN - Hudsonalpha Institute For Biotechnology
item PLOTT, CHRIS - Hudsonalpha Institute For Biotechnology
item VOGEL, JOHN - Lawrence Berkeley National Laboratory
item LEMAUX, PEGGY - University Of California
item MOCKLER, TODD - Danforth Plant Science Center
item GRIMWOOD, JANE - Hudsonalpha Institute For Biotechnology
item SCHMUTZ, JEREMY - Hudsonalpha Institute For Biotechnology
item PRESSOIR, GAEL - Quisqueya University
item MORRIS, GEOFFREY - Kansas State University

Submitted to: bioRxiv
Publication Type: Pre-print Publication
Publication Acceptance Date: 5/13/2021
Publication Date: 5/13/2021
Citation: Muleta, K.T., Felderhoff, T., Winans, N., Walstead, R., Charles, J., Armstrong, J.S., Mamidi, S., Plott, C., Vogel, J.P., Lemaux, P.G., Mockler, T.C., Grimwood, J., Schmutz, J., Pressoir, G., Morris, G.P. 2021. The recent evolutionary rescue of a staple crop depended on over half a century of global germplasm exchange. bioRxiv.

Interpretive Summary: Through genomics analyses of 296 Haitian germplasm and post selection of 767 global accessions, we were able to determine that within a particular genomic loci, a region identified as RMES1, was responsible for the expression of resistance to the sugarcane aphid. All sorghum germplasm identified as resistant was shown to have the RMES1 loci. The resistant source was also found to be the major source for sugarcane aphid resistance in the United States when an epidemic occurred.

Technical Abstract: Rapid environmental change can lead to extinction of populations or evolutionary rescue via genetic adaptation. In the past several years, smallholder and commercial cultivation of sorghum (Sorghum bicolor), a global cereal and forage crop, has been threatened by a global outbreak of an aggressive new biotype of sugarcane aphid (SCA; Melanaphis sacchari). Here we characterized genomic signatures of adaptation in a Haitian sorghum breeding population, which had been recently founded from admixed global germplasm, extensively intercrossed, and subjected to intense selection under SCA infestation. We conducted evolutionary population genomics analyses of 296 post-selection Haitian lines compared to 767 global accessions at 159,683 single nucleotide polymorphisms. Despite intense selection, the Haitian population retains high nucleotide diversity through much of the genome due to diverse founders and an intercrossing strategy. A genome-wide fixation (FST) scan and geographic analyses suggests that adaptation to SCA in Haiti is conferred by a globally-rare East African allele of RMES1, which has also spread to other breeding programs in Africa, Asia, and the Americas. De novo genome sequencing data for SCA resistant and susceptible lines revealed putative causative variants at RMES1. Convenient low-cost markers were developed from the RMES1 selective sweep and successfully predicted resistance in independent U.S. × African breeding lines and eight U.S. commercial and public breeding programs, demonstrating the global relevance of the findings. Together, the findings highlight the potential of evolutionary genomics to develop adaptive trait breeding technology and the value of global germplasm exchange to facilitate evolutionary rescue.