Location: Emerging Pests and Pathogens ResearchTitle: Molecular characterization of a Pseudomonas species associated with disease in Hibiscus
|GONZALEZ-TOBON, JULIANA - Cornell University|
|DIAZ, ALLISON - Cornell University|
|DAUGHTREY, MARGERY - Cornell University|
Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/28/2021
Publication Date: 8/2/2021
Citation: Gonzalez-Tobon, J., Diaz, A., Daughtrey, M., Filiatrault, M.J. 2021. Molecular characterization of a Pseudomonas species associated with disease in Hibiscus. American Phytopathological Society. Plant Health 2021, August 2-6, 2021, Virtual meeting.
Technical Abstract: During the summer of 2019, leaf spots and crinkling symptoms were found on hibiscus (Hibiscus rosa-sinensis) brought into New York from a nursery in Florida. Initial identification using MLST with several housekeeping genes suggested this isolate was a member of the genus Pseudomonas but the species was unclear. This result was consistent with Hibiscus rosa-sinensis being a host to members of the genus Pseudomonas. To definitively determine the species, whole genome sequencing using Nanopore and Illumina sequencing technologies was performed. The genomic sequence of the pathogen shares highest homology to two pathovars of Pseudomonas amygdali. The genus Pseudomonas continually undergoes extensive taxonomic reclassifications. P. amygdali (reported synonyms P. ficuserectae, P. meliae, and P. savastanoi) has been recently proposed to be a separate species, and includes P. amygdali pv. hibisci which is sometimes referred to as P. syringae pv. hibisci, as well. Our data is consistent with this new classification. Additionally, the genome content and structure were compared against widely known Pseudomonas syringae pathovars: tomato DC3000 and B728a. A group of pathogenicity islands containing known effectors from other Pseudomonads were identified. Pathogenicity assays showed that the isolate causes disease in tomato and caused a hypersensitivity reaction in tobacco. The genomic information is being used to better understand the pathogenicity of this pathogen and closely related Pseudomonads that infect floriculture crops.