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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #383861
Research Project: Improvement of Biotic Stress Resistance in Durum and Hard Red Spring Wheat Using Genetics and Genomics

Location: Cereal Crops Research

Title: Genetic mapping of host resistance to the Pyrenophora teres f. maculata isolate 13IM8.3

Author
item ALHASHEL, ABDULLAH - North Dakota State University
item POUDEL, ROSHAN - North Dakota State University
item Fiedler, Jason
item Carlson, Craig
item RASMUSSEN, JACK - North Dakota State University
item BALDWIN, THOMAS - North Dakota State University
item Friesen, Timothy
item BRUEGGEMAN, ROBERT - Washington State University
item Yang, Shengming

Submitted to: Genes, Genomes, Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/17/2021
Publication Date: 9/29/2021
Citation: Alhashel, A., Poudel, R., Fiedler, J.D., Carlson, C.H., Rasmussen, J., Baldwin, T., Friesen, T.L., Brueggeman, R., Yang, S. 2021. Genetic mapping of host resistance to the Pyrenophora teres f. maculata isolate 13IM8.3. Genes, Genomes, Genetics. https://doi.org/10.1093/g3journal/jkab341.
DOI: https://doi.org/10.1093/g3journal/jkab341

Interpretive Summary: Spot form net blotch is one of the most destructive leaf diseases of barley in the US and worldwide. Identification of effective resistance and the corresponding molecular markers is important in controlling this disease. However, barley breeding for spot form net blotch resistance has been hampered by the lack of characterized barley germplasm. Using two mapping populations, we detected a total of ten genetic loci contributing to spot form net blotch resistance, including a major quantitative trait locus. User-friendly markers linked to the identified loci were also designed to assist breeding selection. Therefore, our study provides the critical germplasm and the tools for breeders to incorporate this source of resistance into breeding lines to improve resistance to spot form net blotch of barley.

Technical Abstract: Spot form net blotch (SFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata (Ptm), is a foliar disease of barley that results in significant yield losses in major growing regions worldwide. Understanding the host-parasite interactions between pathogen virulence/avirulence genes and the corresponding host susceptibility/resistance genes is important for the deployment of genetic resistance against SFNB. Two recombinant inbred mapping populations were developed to characterize genetic resistance/susceptibility to the Ptm isolate 13IM8.3, which was collected from Idaho (ID). This isolate exhibited a distinct virulence profile from others collected from three states in the US. An Illumina Infinium array was used to produce a genome wide marker set. Quantitative trait loci (QTL) analysis identified ten significant resistance/susceptibility loci, with two of the QTL being common to both populations. One of the QTL on 5H appears to be novel, while the remaining loci have been reported previously. SNPs closely linked to or delimiting the significant QTL have been converted to user-friendly markers. Loci and associated molecular markers identified in this study will be useful in the deployment of genetic resistance to SFNB in barley.