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ARS Home » Southeast Area » Tifton, Georgia » Crop Genetics and Breeding Research » Research » Publications at this Location » Publication #383569

Research Project: Improvement of Genetic Resistance to Multiple Biotic and Abiotic Stresses in Peanut

Location: Crop Genetics and Breeding Research

Title: High resolution genetic and physical mapping of a peanut spotted wilt disease resistance locus, PSWD-1, to Tomato spotted wilt virus (TSWV)

item Guo, Baozhu
item ZHAO, C - Shandong Academy Of Agricultural Sciences
item MAO, C - Shandong Academy Of Agricultural Sciences
item WANG, X - Shandong Academy Of Agricultural Sciences
item FOUNTAIN, J - Mississippi State University
item KORANI, W - Hudsonalpha Institute For Biotechnology
item CLEVENGER, J - Hudsonalpha Institute For Biotechnology
item AGARWAL, G - University Of Georgia
item WANG, H - University Of Georgia
item ALI, E - University Of Georgia
item CULBREATH, A - University Of Georgia
item Wang, Ming

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary: not required.

Technical Abstract: Quantitative trait loci (QTLs) for resistance to Tomato spotted wilt virus (TSWV) in peanut were previously mapped on chromosome A01 in the recombinant inbred line (RIL) mapping population derived from SunOleic 97R X NC94022 using SSR markers and whole genome resequencing. Using the recently released complete genome sequences for both diploid and tetraploid peanut, and the high-density peanut SNP arrays (58K ‘Axiom_ Arachis_v1’ and the haplotype-based 48K ‘Axiom_ Arachis_v2’ arrays), we developed high-resolution genetic and physical maps of the peanut TSWV resistance locus PSWD-1. QTL analysis of five years’ TSWV disease ratings identified two major QTLs on chromosome A01 explaining 41.43% and 43.69% of phenotypic variance, within 3.7 cM and 0.3 cM in the same region of the genetic maps, respectively, using these two versions of peanut SNP arrays. These QTL intervals corresponded to a 490-kb physical interval. Comparison of the genetic map and the reference genome revealed a ‘cold spot’ region from 12,040,521 bp to 12,528,768 bp (488 kb) recombination being observed only in two RILS, S1 and S17, which also had clear, contrasting TSWV phenotypes. The recombination “cold spot” was confirmed using re-sequencing data from these two RILs. Therefore, based on the genetic and physical maps we named the resistance locus as the peanut spotted wilt disease 1 (PSWD-1) resistance locus. Within this locus there were three candidate genes including an NBS-LRR disease resistance gene, a glutamate receptor, and an MLO-like protein. The strongest candidate resistance gene is the TIR-NBS-LRR R-gene with the gene ID arahy.1PK53M, which is a prime candidate for future functional characterization and use in genetic improvement of peanut resistance to TSWV.