Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2021
Publication Date: N/A
Interpretive Summary: n/a
Technical Abstract: Tissue culture derived plantlets have to be adapted to outside environmental conditions before transplanting to field. They need to be adjusted to growing in a lower RH and higher light intensity than in vitro. Before planting into a soil mixture, the culture medium should be washed off from the plantlet roots and the shoot base (rhizome) under running water until all agar is removed. A successful acclimation procedure for Musa (banana) plantlets involves dipping the roots in a fungicide [e.g., Methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate] and in a root-inducing auxin (IBA and NAA) product. The medium-free and treated plantlets are transferred to small pots with a sterilized substrate and covered with a clear plastic cup or misted intermittently; the misting time is gradually reduced as the plants grow. After ca. 2 weeks, the plants are fertilized with a 1/3 strength of a 20-20-20 soluble fertilizer and the plastic cup is removed. Following a 3-4 months growing in a greenhouse, 1 to 2 ft tall plants with 3-4 leaves are sprayed with an anti-transpirant and planted in the field where normal agronomic practices for banana cultivation are applied.