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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #380447
Research Project: Characterization of Antigens, Virulence Markers, and Host Immunity in the Pathogenesis of Johne’s Disease

Location: Infectious Bacterial Diseases Research

Title: Complete genome sequence of a type III ovine strain of Mycobacterium avium subspecies paratuberculosis

Author
item Bannantine, John
item Bayles, Darrell
item BIET, FRANCK - Université François-Rabelais De Tours

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/12/2021
Publication Date: 3/11/2021
Citation: Bannantine, J.P., Bayles, D.O., Biet, F. 2021. Complete genome sequence of a type III ovine strain of Mycobacterium avium subspecies paratuberculosis. Microbiology Resource Announcements. 10. https://doi.org/10.1128/MRA.01480-20.
DOI: https://doi.org/10.1128/MRA.01480-20

Interpretive Summary: Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease in farmed livestock, including cattle, sheep and goats. The genome of the first type III sheep strain of MAP was sequenced and assembled to complete closure. Previously, type II bovine strains and a type I sheep strain were sequenced to completion and available publicly, but no type III strains. This new information will unlock the genetic diversity this exists within MAP to help us understand host preferences and evolution of this veterinary pathogen. Finally, this new sequence will better inform diagnostic targets.

Technical Abstract: The complete genome sequence of the first type III strain of Mycobacterium avium subspecies paratuberculosis was determined. The genome size for this pathogen of sheep is 4,895,755 bp with no plasmid DNA. The chromosome contains 19 copies of the hallmark IS900 element, which is routinely used to identify this subspecies.