|LIU, FEIFEI - University Of Hawaii|
|MALLICK, SUDIPTA - University Of Hawaii|
|O'DONNELL, TIMOTHY - University Of Hawaii|
|ROUZIMAIMAITI, R - University Of Hawaii|
|LUO, YUHENG - University Of Hawaii|
|SUN, RUI - University Of Hawaii|
|WONGWIWATTHANANUKIT, SUPAKIT - University Of Hawaii|
|DATE, ABHIJIT - University Of Hawaii|
|SILVA, DANE - Ohana Learning Institute|
|WILLIAMS, PHILIP - University Of Hawaii|
|CHANG, LENG CHEE - University Of Hawaii|
Submitted to: Molecules
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/16/2022
Publication Date: 5/19/2022
Citation: Liu, F., Mallick, S., O'Donnell, T.J., Rouzimaimaiti, R., Luo, Y., Sun, R., Wall, M.M., Wongwiwatthananukit, S., Date, A., Silva, D., Williams, P.G., Chang, L. 2022. Courmarinolignans with reactive oxygen species (ROS) and NK-¿B inhibitory activities from the roots of Walteria indica. Molecules 2022, 27, 3270. https://doi.org/10.3390/molecules27103270.
Interpretive Summary: Walteria indica is a short-lived shrub widespread in subtropical and tropical regions that has been used traditionally to treat asthma, inflammation, neuralgia and pain. The plant parts are known to contain biologically active plant compounds such as polyphenols, flavonoids, coumarins and quinolone alkaloids. In this study, human embryonic kidney cells were used to evaluate the anti-inflammatory effects of phytochemicals isolated from Waltheria indica roots. We report the isolation, structure elucidation, and anti-inflammatory activities of seven new coumarinolignans, along with five known analogues. All compounds tested were found to have inhibitory response to some extent, but two new compounds were most potent showing 52.8% and 54% inhibition, respectively.
Technical Abstract: Seven new coumarinolignans, walthindicins A-F (1a-5, 7), along with five known analogues (6, 8-11), were isolated from the roots of Waltheria indica. The structures of the new compounds were elucidated by detailed nuclear magnetic resonance (NMR), circular dichroism (CD) with extensive computational support, and mass spectroscopic data interpretation. All compounds were tested for their antioxidant activity in Human Cervical Cancer cells (HeLa cells). Compounds 1a and 6 showed higher reactive oxygen species (ROS) inhibitory activity at 20µg/mL when compared with other natural compound-based antioxidants like ascorbic acid. Considering the role of ROS in nuclear-factor kappa B activation, both compounds 1a and 6 were evaluated for nuclear-factor kappa B inhibitory activity and showed a concentration-dependent inhibition in Human Embryonic Kidney 293 cells (Luc-HEK-293) cells.