Location: Cereal Crops ResearchTitle: Antagonistic regulation of auxiliary bud outgrowth by the branched genes in tobacco
|DING, NA - University Of Kentucky|
|QIN, QIULIN - University Of Kentucky|
|WU, XIA - University Of Kentucky|
|MILLER, ROBERT - University Of Kentucky|
|ZAITLIN, DAVID - University Of Kentucky|
|LI, DANDAN - University Of Kentucky|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/28/2019
Publication Date: 1/13/2020
Citation: Ding, N., Qin, Q., Wu, X., Miller, R., Zaitlin, D., Li, D., Yang, S. 2020. Antagonistic regulation of auxiliary bud outgrowth by the branched genes in tobacco [abstract]. Plant and Animal Genome Conference XXVIII. PE0502.
Interpretive Summary: .
Technical Abstract: As a key signaling integrator of shoot branching, BRANCHED 1 (BRC1) coordinates and is orchestrated by endogenous and environmental signals involved in the regulation of axillary bud outgrowth. Using CRISPR site-directed mutagenesis and overexpression assays, we characterized the regulatory roles of five BRC gene members in tobacco. We show that lateral branching is negatively regulated by NtBRC1A-1, 1B-1, and 1B-2, but was unexpectedly promoted by NtBRC2A. The NtBRC1A-2 gene seems not to be required for the regulation of axillary bud outgrowth. Suppression of bud growth may be attained by direct binding of NtBRC proteins to Tassels Replace Upper Ears 1 (TRU1) genes, as evidenced by RNAi and CRISPR-mediated gene editing. The BRC2 gene, which is thought to be unnecessary for branch development, probably confers a dominant negative effect by interfering with the branching-inhibitory BRC1 genes. However, the molecular mechanism of NtBRC2A action still needs to be further addressed. Our results may indicate that there is a blind spot when using genetic tools, such as CRISPR and RNAi, in the functional characterization of members of a gene family.