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Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

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Title: Identification of differentially expressed proteins involved in plant resistance to Aphid

Author
item HUANG, JIAN - Oklahoma State University
item Huang, Yinghua

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/27/2019
Publication Date: 1/10/2020
Citation: Huang, J., Huang, Y. 2020. Identification of differentially expressed proteins involved in plant resistance to Aphid. [Abstract}. Plant & Animal Genome Conference XXVIII. Poster No. PO0039. Available:https://pag.confex.com/pag/xxviii/meetingapp.cgi/Paper/40796

Interpretive Summary:

Technical Abstract: Greenbug, Schizaphis graminum (Rondani), is known as a major insect pest of small grains throughout the world, including wheat, barley and sorghum. Resistance sources in those crops have been identified and are being widely used to manage this important aphid pest; however, little is known about the mechanism of host plant resistance to greenbug. The previous studies demonstrated that resistant plants initiated their innate defense systems in response to greenbug feeding based on the transcriptional profiles developed in sorghum plants. In this study, we undertook proteomic analysis of aphid-challenged sorghum seedlings to compare expression changes at the protein level between greenbug resistant and susceptible lines, PI550610 (resistant) and BTx623 (susceptible) in order to elucidate molecular mechanisms of insect resistance in sorghum. Seedlings of two sorghum lines were infested with freshly prepared virulent greenbug aphids and leaf tissues were collected on fifth day after infestation. Then total proteins were extracted for the leaf samples and analyzed in parallel using two dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS). At least 108 proteins spots reproducibly detected on the protein gels. Of these, comparing resistant and susceptible lines, twenty differentially expressed proteins were identified by MALDI-TOF/MS. Putative function of some differentially expressed proteins is inferred from metabolic processes to plant defense, while others showed as novel genes that need to be confirmed experimentally. We believe that those proteomic data provide new insights on plant defensive responses to greenbug attack in sorghum.