Location: Southern Horticultural ResearchTitle: Genetic diversity and pedigree analysis of muscadine grapes (Vitis rotundifolia) using SSR markers
|CAO, SHANSHAN - University Of Georgia|
|GUNAWAN, GUNAWATI - University Of Georgia|
|CONNER, PATRICK - University Of Georgia|
|MCGREGOR, CECILIA - University Of Georgia|
Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/13/2019
Publication Date: 3/19/2020
Citation: Cao, S., Stringer, S.J., Gunawan, G., Conner, P.J., Mcgregor, C. 2020. Genetic diversity and pedigree analysis of muscadine grapes (Vitis rotundifolia) using SSR markers. HortScience. 14(3):143-151. https://doi.org/10.21273/JASHS04856-20.
Interpretive Summary: Muscadine grape breeding over the last century has created a large number of cultivars that are normally identified by comparing morphological traits and checking breeding records which are sometimes ambiguous and unauthentic. This study used simple sequence repeat (SSR) markers to generate fingerprinting profiles to identify muscadine cultivars and verify their reported pedigrees. Eighty-one unique Muscadinia accessions were identified and a core set of five SSR markers was able to distinguish them all.
Technical Abstract: Muscadine grape (Vitis rotundifolia) was the first native North American grape to be domesticated. Over the last century breeding programs have created a large collection of muscadine cultivars. They are normally identified by comparing morphological traits and checking breeding records, which are often ambiguous and can be unauthentic. In this study, simple sequence repeat (SSR) markers were used to generate DNA fingerprinting profiles to identify muscadine cultivars and verify their reported pedigrees. Eighty-nine unique genotypes were differentiated out of 190 accessions using twenty SSRs from thirteen linkage groups. Eighty-one unique Muscadinia accessions were identified and a core set of five SSR markers was able to distinguish all of them. Nine cultivars were found with misidentified accessions, and five previously unknown accessions were matched with cultivars in the dataset. The profile of twelve cultivars was not consistent with their reported parent-progeny relationships. Genetic diversity was analyzed at four levels: all V rotundafolia cultivars (N=68), current cultivars (n=40), historical cultivars (n=29), and wild V. rotundifolia accessions (N=9). Results showed there was substantial genetic diversity in both wild and historically cultivated muscadines. The Principal Coordinate Analysis (PCoA) shows clear separation among subgenus Vitis cultivars, wild muscadine selections, and cultivated muscadines with PCoA1 and OCoA2 explaining 11.1% and 9.3% of the total variation, respectively.