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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #375482

Research Project: Antibiotic Alternatives for Controlling Foodborne Pathogens and Disease in Poultry

Location: Poultry Production and Product Safety Research

Title: Thymosin Beta 4 dynamics during chicken enteroid development

item ACHARYA, MOHAN - University Of Arkansas
item LIYANAGE, ROHANA - University Of Arkansas
item GUPTA, ANAMIKA - University Of Arkansas
item ARSI, KOMALA - University Of Arkansas
item Donoghue, Ann - Annie
item LAY, JACKSON - University Of Arkansas
item Rath, Narayan

Submitted to: Molecular and Cellular Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/25/2020
Publication Date: 12/10/2020
Citation: Acharya, M., Liyanage, R., Gupta, A., Arsi, K., Donoghue, A.M., Lay, J.O., Rath, N.C. 2020. Thymosin Beta 4 dynamics during chicken enteroid development. Molecular and Cellular Biochemistry. 476:1303–1312.

Interpretive Summary: Physiological or pathological changes in the tissues entail many differences including changes in their structural and functional constituent proteins. Using an experimental model of intestinal healing, we found significant changes in a small protein named thymosin beta 4 which regulates cellular proteins that maintain their structure, migration, orientation, wellbeing, and wound healing process necessary for intestinal repair.

Technical Abstract: The sheared avian intestinal villus-crypts exhibit high tendency to self-repair and develop enteroids in culture. Presuming that this transition process can involve differential biomolecular changes, we employed Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (MALDI-TOF-MS) to find whether there were differences in the spectral profiles of sheared villi versus the enteroids, assessed in the mass range of 2~18 kDa. The results showed substantial differences in the intensities of various spectral peaks a particular one corresponding to the mass of 4963 Da which was significantly low in the sheared villus-crypts compared with the enteroids. Based on our previous results with other avian tissues and further molecular characterization by LC-ESI-IT-TOF-MS, and multiple reaction monitoring (MRM), the peak was identified as thymosin Beta 4 (TB4), a ubiquitously occurring regulatory peptide, implicated in wound healing process. The identity of the peptide was further confirmed by immunohistochemistry which showed it to be present in a very low level in the sheared but replete in healed enteroids. Since TB4 sequesters G-actin, preventing its polymerization to F-actin, we compared the changes in F-actin levels by immunohistochemical localization that did not appear to be different between the sheared villi and enteroids. We propose that depletion of TBeta4 likely precedes villous repair process. The possible mechanism for the differences in TB4 profile in relation to the healing of the villus-crypts to developing enteroids is discussed.