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ARS Home » Southeast Area » Poplarville, Mississippi » Southern Horticultural Research » Research » Publications at this Location » Publication #374084

Research Project: Production and Disease and Pest Management of Horticultural Crops

Location: Southern Horticultural Research

Title: Draft genome assembly of Passalora sequoiae a needle blight pathogen on leyland cypress

Author
item Copes, Warren
item IBARRA CABALLERO, JORGE - Colorado State University
item Babiker, Ebrahiem
item STEWART, JANE - Colorado State University
item Orner, Valerie
item WINDHAM, ALAN - University Of Tennessee
item Arias De Ares, Renee

Submitted to: BMC Research Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/5/2020
Publication Date: 11/4/2020
Citation: Copes, W.E., Ibarra Caballero, J., Babiker, E.M., Stewart, J.E., Orner, V.A., Windham, A.S., Arias De Ares, R.S. 2020. Draft genome assembly of Passalora sequoiae a needle blight pathogen on leyland cypress. BMC Research Notes. 13:505. https://doi.org/10.1186/s13104-020-05328-3.
DOI: https://doi.org/10.1186/s13104-020-05328-3

Interpretive Summary: This is report of the whole genome of Passalora sequoiae isolate 9LC2 that was recovered from blighted twig samples on Leyland cypress in southern Mississippi. The genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing to produce a high-quality draft assembly estimated at 31,768,716 bp. The whole genome provides a resource for future applications in monitoring the seasonal dispersal patterns of the pathogen, investigating genotype diversity of traits such as fungicide resistance and in cypress breeding programs.

Technical Abstract: Objectives: Passalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses to both the ornamental plant nursery and Christmas tree producers. The objective was to generate a high-quality draft assembly of the whole genome as a resource for future applications such as temporal and spatial dispersal studies of the pathogen, and to investigate genotype diversity relevant in fungicide resistance studies and cypress breeding programs. Data description: We report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress 'Leighton Green' in 2017 in southern Mississippi. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine alignment consistency. Based on a total of 44 contigs with 722 kb average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 x coverage and were used to confirm accuracy of the consensus sequences.