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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #373320

Research Project: Characterization of Molecular Networks in Diseases Caused by Emerging and Persistent Bacterial Plant Pathogens

Location: Emerging Pests and Pathogens Research

Title: Complete genome sequence of the necrotrophic plant-pathogenic bacterium Pectobacterium brasiliense 1692

Author
item LIU, YINGYU - Cornell University - New York
item Filiatrault, Melanie

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/24/2020
Publication Date: 3/19/2020
Citation: Liu, Y., Filiatrault, M.J. 2020. Complete genome sequence of the necrotrophic plant-pathogenic bacterium Pectobacterium brasiliense 1692. Microbiology Resource Announcements. 9:11. https://doi.org/10.1128/MRA.00037-20.
DOI: https://doi.org/10.1128/MRA.00037-20

Interpretive Summary: Blackleg disease has resulted in significant economic losses and continues to devastate the potato industry. Pectobacterium brasiliense is one of the most aggressive Pectobacterium species known to date causing blackleg of potatoes in the field and soft rot during post-harvest storage. Here, we report the complete genome sequence of P. brasiliense 1692 that was originally isolated from potato in Brazil and responsible for causing soft rot disease on multiple crops worldwide. This sequence is now publicly available and is being used to help better understand blackleg and soft rot diseases of potatoes.

Technical Abstract: We report the complete and annotated genome sequence of Pectobacterium brasiliense 1692, a Gram-negative enterobacterium that can cause soft rot disease on many plant hosts. Whole-genome sequencing technology capable of generating long reads (Nanopore) was used to obtain a complete scaffold of the genome and short reads (Illumina) were used to polish the assembled genome. The final polished P. brasiliense 1692 genome was 4,851,982'bp long with 52.15% GC content. It contained 4,310 genes in total, with 4,139 protein-coding genes, 65 pseudogenes, 8, 7, and 7 rRNA-coding sequences (5S, 16S, and 23S rRNAs), 77 tRNAs, and 7 noncoding RNAs. Two clustered regularly interspaced short palindromic repeat (CRISPR) arrays were found in the genome.