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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #371695
Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Characterization, detection and management of emerging tomato brown rugose fruit virus infecting greenhouse tomatoes in the U.S.

Author
item Chanda, Bidisha
item RIVERA, YAZMÍN - Animal And Plant Health Inspection Service (APHIS)
item NUNZIATA,, SCHYLER - Animal And Plant Health Inspection Service (APHIS)
item GALVEZ, MARCO - Animal And Plant Health Inspection Service (APHIS)
item Gilliard, Andrea
item Ling, Kai-Shu

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2020
Publication Date: 10/1/2020
Citation: Chanda, B., Rivera, Y., Nunziata,, S., Galvez, M., Gilliard, A.C., Ling, K. 2020. Characterization, detection and management of emerging tomato brown rugose fruit virus infecting greenhouse tomatoes in the U.S.. Phytopathology. https://doi.org/10.1094/PHYTO-110-7-S1.1.
DOI: https://doi.org/10.1094/PHYTO-110-7-S1.1

Interpretive Summary:

Technical Abstract: Tomato brown rugose fruit virus (ToBRFV), first identified in 2014 in the Middle East, has caused serious outbreaks in greenhouse tomatoes of many countries in Asia, Europe and North America. With its seed-borne, easy mechanical transmission and resistance breaking to the popular Tm2^2 gene, ToBRFV could potentially cause serious economic losses to tomato industries worldwide if not managed timely. In the U.S., the first ToBRFV outbreak on tomato was reported in 2018 in a greenhouse facility in California. In the current study, using high throughput sequencing, a complete genome sequence of the US isolate of ToBRFV was obtained. The high nucleotide sequence identity (99%) to other known ToBRFV isolates around the world suggests a high possibility in sharing the same or similar origin. In a comparative evaluation of potential host ranges among tomato-infecting tobamoviruses, despite of many similarities, some unique host species were also identified for each respective virus. Due to cross serological reactivity among tomato-infecting tobamoviruses, a species-specific real-time RT-PCR was developed for ToBRFV. Disinfectants that are capable of deactivating virus infectivity to prevent mechanical transmission were evaluated and several promising disinfectants were identified.Finally, nearly 500 accessions of tomato germplasm and cultivars were screened for their potential in resistance against ToBRFV.