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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #371231

Research Project: Efficient and Effective Preservation and Management of Plant and Microbial Genetic Resource Collections

Location: Agricultural Genetic Resources Preservation Research

Title: Collecting and maintaining exceptional species in tissue culture and cryopreservation

item PENCE, VALERIE - Cincinnati Zoo & Botanical Garden
item WESTWOOD, MURPHY - Morton Arboretum
item MASCHINSKI, JOYCE - Center For Plant Conservation (CPC)
item POWELL, CHRISTY - San Diego Zoo
item SUGII, NELLIE - Lyon Arboretum
item FISH, DIANA - Center For Plant Conservation (CPC)
item MCGUINNESS, JULIANNE - North American Orchid Conservation Center (NAOCC)
item RAVEN, PAT - Missouri Botanical Garden
item DUVAL, JULIAN - San Diego Botanic Garden
item HERRERA-MISHLER, TOMAS - Balboa Park Conservancy
item LOVE, ANDREW - Center For Plant Conservation (CPC)
item Walters, Christina
item HORN, CHRISTA - San Diego Zoo
item TAYLOR, MATT - Longwood Gardens
item OTT, THOMAS - Center For Plant Conservation (CPC)
item KOEHLER, STEVEN - Missouri Botanical Garden
item HORNING, MATT - Us Forest Service (FS)

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/10/2020
Publication Date: 8/14/2019
Citation: Pence, V., Westwood, M., Maschinski, J., Powell, C., Sugii, N., Walters, C.T., et al. 2019. Collecting and maintaining exceptional species in tissue culture and cryopreservation. In: Falk, D., Holsinger, K., Wieland, G., Olwell, P., et al., editors. CPC Best Plant Conservation Practices to Support Species Survival in the Wild. Washington, D.C.: Island Press. p. 4-21.

Interpretive Summary: n/a

Technical Abstract: Exceptional plants are those that cannot be conserved long-term using conventional seed banking methods. This includes species producing few or no seeds for banking, species producing seeds that are intolerant of desiccation and freezing, or seeds that can tolerate drying, but not freezing, or species that produce seeds that may only tolerate storage at -20C for less than 10 years. For ex situ conservation, such species require methods alternative to conventional storage, such as cryopreservation or in vitro methods. Many of the world's plant species may fit these storage categories. The primary purpose of a conservation collection for an exceptional species is to support the species survival and reduce its extinction risk. Therefore accurate records of provenance, differentiated maternal lines and diverse genetic representation are prerequisites.