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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #370080
Research Project: Improvement of Biotic Stress Resistance in Durum and Hard Red Spring Wheat Using Genetics and Genomics

Location: Cereal Crops Research

Title: Molecular characterization of Hessian fly resistance genes in Aegilops tauschii

Author
item LONG, YUMING - North Dakota State University
item ZHANG, QIJUN - Orise Fellow
item ANDERSON, KIRK - North Dakota State University
item YU, GUOTAI - North Dakota State University
item ARORA, SANU - John Innes Center
item STEUERNAGEL, BURKHARD - John Innes Center
item LUO, MING-CHENG - University Of California, Davis
item CAI, XIWEN - North Dakota State University
item Gu, Yong
item Wuddineh, Wegi
item Faris, Justin
item Chen, Ming-Shun
item Xu, Steven
item WULFF, BRANDE B - John Innes Center
item LAGUDAH, EVANS - Csiro, Black Mountain Laboratories
item HARRIS, MARION - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/16/2019
Publication Date: 1/11/2020
Citation: Long, Y., Zhang, Q., Anderson, K.M., Yu, G., Arora, S., Steuernagel, B., Luo, M., Cai, X., Gu, Y.Q., Wuddineh, W., Faris, J.D., Chen, M., Xu, S.S., Wulff, B.H., Lagudah, E.S., Harris, M.O. 2020. Molecular characterization of Hessian fly resistance genes in Aegilops tauschii [abstract]. Plant and Animal Genome Conference XXVIII. W809.

Interpretive Summary: .

Technical Abstract: Aegilops tauschii Cosson, the D-genome donor of bread wheat (Triticum aestivum L.), provides a vast reservoir of genetic variation for wheat improvement in disease and insect resistance. For the resistance to Hessian fly (Mayetiola destructor Say), six resistance genes (H13, H22, H23, H24, H26, and H32) have been identified in Ae. tauschii. The objectives of this study were to identify and characterize Hessian fly resistance genes from a worldwide collection of Ae. tauschii accessions deposited in the USDA-ARS National Small Grains Collection and Wheat Genetics Resource Center. To identify the resistance, we evaluated approximately 600 Ae. tauschii accessions for reactions to the Hessian fly biotype Great Plains and identified 90 resistant accessions. To identify potentially novel genes, we conducted fine mapping of the resistance genes in three Ae. tauschii accessions (CIae 25, RL 5271, and TA 2377) using the large F2 populations derived from their respective crosses with a susceptible Ae. tauschii accession AL8/78. Two tightly-linked resistance genes were identified from CIae 25 and mapped to the H26 region (3DL). The resistance genes in RL 5271 and TA 2377 were both mapped onto the genomic region harboring H13 (6DS). By surveying the genomic sequences of approximately 260 Ae. tauschii accessions, we identified several candidate genes in RL 5271 and TA 2377. Sequence analysis revealed that one of the candidate genes is located in the same region as the H13 gene in the wheat genotype Molly, with two single-nucleotide mutations in comparison with the H13 candidate, indicating that the Hessian fly resistance gene in RL 5271 and TA 2377 is likely a new haplotype of H13. This study also demonstrated that several simple sequence repeat (SSR) and semi-thermal asymmetric reverse PCR markers that co-segrgate with or are tightly linked to these targeted genes are useful for developing resistant cultivars and cloning resistance genes for Hessian fly.