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ARS Home » Midwest Area » Madison, Wisconsin » Cereal Crops Research » Research » Publications at this Location » Publication #369615

Research Project: Identifying the Next Generation of Malting Barley Through Improved Selection Criteria and Quality Analysis of Breeding Lines

Location: Cereal Crops Research

Title: Mutations in HvMKK3 and HvAlaAT1 genes affect barley pre-harvest sprouting and after-ripened seed dormancy

item VETCH, JUSTIN - Montana State University
item SHERMAN, JAMIE - Montana State University
item MARTIN, JOHN - Montana State University
item GIROUX, MICHAEL - Montana State University
item Walling, Jason

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/13/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Preharvest sprouting (PHS) is a natural phenomenon that negatively impacts various crops across the globe when late season rainfall causes seeds to germinate prior to harvest. PHS damage could be mitigated by incorporation of genetic alleles that impart desired levels of dormancy at specific times in grain maturity. Toward this goal, 114 barley varieties were assessed for dormancy at physiological maturity and in after-ripened grains. Three genes previously associated with dormancy in barley or wheat (HvAlaAT1, HvMKK3, and HvMFT) were sequenced from all lines and assessed for allelic diversity. The resulting alleles were assessed for association with dormancy at each of the maturity time points. In total, seven missense mutations were discovered across the three genes. Here we report missense mutations in both HvAlaAT1 and HvMKK3 that significantly affect dormancy levels in barley.