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ARS Home » Southeast Area » Stoneville, Mississippi » Southern Insect Management Research » Research » Publications at this Location » Publication #366542
Research Project: Integrated Insect Pest and Resistance Management on Corn, Cotton, Sorghum, Soybean, and Sweet Potato

Location: Southern Insect Management Research

Title: In silico discovery of genes encoding insecticide targets and detoxifying enzymes in Brevicoryne brassicae and Lipaphis erysimi

Author
item LI, WENHONG - Guizhou Academy Of Agricultural Sciences
item Zhu, Yu Cheng
item LI, FENGLIANG - Guizhou Academy Of Agricultural Sciences
item CHENG, YING - Guizhou Academy Of Agricultural Sciences
item JIN, JIANXIU - Guizhou Academy Of Agricultural Sciences
item ZHOU, YUHANG - Guizhou Academy Of Agricultural Sciences
item HE, YUEPING - Huazhong Agricultural University

Submitted to: Journal of Asia-Pacific Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/3/2019
Publication Date: 1/10/2020
Citation: Li, W., Zhu, Y., Li, F., Cheng, Y., Jin, J., Zhou, Y., He, Y. 2020. In silico discovery of genes encoding insecticide targets and detoxifying enzymes in Brevicoryne brassicae and Lipaphis erysimi. Journal of Asia-Pacific Entomology. 23(1):159-166. https://doi.org/10.1016/j.aspen.2019.12.002.
DOI: https://doi.org/10.1016/j.aspen.2019.12.002

Interpretive Summary: The aphid complex (cabbage aphid, mustard aphid, and green peach aphid) in cruciferous crops are extremely similar in appearance but they however showed differences in their levels of susceptibilities to various insecticides. The lacking of genetic information on cabbage and mustard aphids limits our understanding of resistance status among aphid species at the molecular level. Based on the mining of the public molecular and genetic data, we identified a number of genes encoding three detoxifying enzymes, carboxylesterases (containing acetylcholinesterases), cytochrome P450s, and glutathione S-transferases. Several insecticide targets were also identified, such as acetylcholinesterase, nicotinic acetylcholine receptor, '-aminobutyric acid gated ion channel, glutamate receptor, the voltage-gated sodium channel, ryanodine receptor, transient receptor potential vanilloid channel, and inward-rectifier potassium channel. Our study provides genetic information for understanding the diversity of insecticide resistance occurring among cruciferous aphids, which could meaningfully contribute towards complementing future related studies.

Technical Abstract: The aphid complex (Brevicoryne brassicae, Lipaphis erysimi and Myzus persicae) in cruciferous crops are extremely similar in appearance but they however showed differences in their levels of susceptibilities to various insecticides. The lacking of genetic information on B. brassicae and L. erysimi limits our understanding of resistance status among aphid species at the molecular level. Based on the mining of the public transcriptome data, we identified a number of genes encoding three detoxifying enzymes, carboxylesterases (containing acetylcholinesterases), cytochrome P450s, and glutathione S-transferases. Several insecticide targets were also identified, such as acetylcholinesterase, nicotinic acetylcholine receptor, '-aminobutyric acid gated ion channel, glutamate receptor, the voltage-gated sodium channel, ryanodine receptor, transient receptor potential vanilloid channel, and inward-rectifier potassium channel. Our study provides genetic information for understanding the diversity of insecticide resistance occurring among cruciferous aphids, which could meaningfully contribute towards complementing future related studies.