Location: Cereal Crops ResearchTitle: Molecular regulation and genetic manipulation of alkaloid accumulation in tobacco plants
|GUO, YONGFENG - Chinese Academy Of Agricultural Sciences|
|HIATT, ERNIE - Rjr Tobacco Company|
|BONNET, CHRISTELLE - Japan Tobacco International|
|KUDITHIPUDI, CHENGALRAYAN - Altria Client Services|
|SHI, HONGZHI - Henan Agricultural University|
|PATRA, BARUNAVA - University Of Kentucky|
|LEWIS, RAMSEY - North Carolina State University|
|DORLHAC DE BORNE, FRANCOIS - Imperial Tobacco|
|LUSSO, MARCOS - Altria Client Services|
|BERGER, IRVING - British American Tobacco(BRAZIL)|
|ZHANG, HONGBO - Chinese Academy Of Agricultural Sciences|
|GILLES, TIJS - British American Tobacco(BRAZIL)|
|XU, DONGMEI - Altria Client Services|
Submitted to: Bioactive Natural Products
Publication Type: Book / Chapter
Publication Acceptance Date: 8/31/2020
Publication Date: 8/1/2021
Citation: Guo, Y., Hiatt, E., Bonnet, C., Kudithipudi, C., Shi, H., Patra, B., Lewis, R.S., Dorlhac De Borne, F., Lusso, M., Berger, I., Zhang, H., Yang, S., Gilles, T., Xu, D. 2021. Molecular regulation and genetic manipulation of alkaloid accumulation in tobacco plants. In: Rahman, A., editor. Studies in Natural Products Chemistry. Amsterdam, Netherlands: Elsevier B.V. p. 119-149. https://doi.org/10.1016/B978-0-12-819489-8.00006-5.
Interpretive Summary: The FDA recently announced their intention to mandate a reduction in nicotine in commercial tobacco products to “non-addictive levels”. Therefore, decreasing nicotine concentrations in tobacco leaves is of practical importance for tobacco breeders and industry. Although low levels of nicotine can be obtained through a disruption of genes involved in nicotine biosynthesis, reduction in nicotine accumulation results in adverse effects on leaf yield and quality. In this paper, we reviewed genetic pathways for nicotine biosynthesis, conversion and transport, and proposed potential means to decrease nicotine levels in tobacco plants. This paper sheds light on ways to develop tobacco varieties with both ultra-low nicotine levels and good agronomic characteristics, providing novel strategies for breeders and the tobacco industry.
Technical Abstract: Nicotine and related pyridine alkaloids of the tobacco plant are synthesized in the root and subsequently translocated via the xylem to the leaf and other aerial tissues, where they are primarily stored in vacuoles as a defensive chemical against herbivorous insects. Tobacco alkaloid accumulation depends on both biosynthesis and transport, which can be influenced by environmental conditions, phytohormones. and developmental status. Jasmonate signaling and the ERF transcription factors are known to play predominant regulatory roles. Genetic manipulation of alkaloid accumulation via traditional breeding, mutation breeding, and genetic engineering has been effective, but adverse effects on yields and cured leaf quality are frequently observed. Prospects for developing tobacco cultivars with ultra-low alkaloid levels to meet future potential regulation of combustible cigarette nicotine content are discussed.