|APPALA, KEERTHI - Western Kentucky University|
|CONTE, ERIC - Western Kentucky University|
|KASUMBA, JOHN - Western Kentucky University|
|CARLISLE, ANNE - Western Kentucky University|
Submitted to: Proceedings of Pittcon Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/16/2018
Publication Date: 3/19/2019
Citation: Appala, K., Conte, E., Kasumba, J., Agga, G.E., Loughrin, J.H., Carlisle, A. 2019. Quantification of tylosin antibiotics and antibiotic resistance genes in cattle waste. Proceedings of Pittcon Meeting. Poster No. 890-1P.
Technical Abstract: Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 people are estimated to die because of antibiotic resistance every year. New drugs are coming into the market but are at the threat of developing resistance. One of the reasons for the development of antibiotic resistance is the overuse of antibiotics in the livestock. Tylosin is a macrolide antibiotic found naturally as a fermentation product of Streptomyces fradiae and is mainly used in promoting growth and treating infections in animals. Tylosin acts by inhibiting protein synthesis in bacteria. In cattle, tylosin is used for treating the bovine respiratory complex, foot-rot and calf diphtheria while in swine it is used to treat swine arthritis, swine pneumonia and swine erysipelas. The products from livestock, treated with antibiotics such as milk, meat (Chicken, pork, cattle beef), excreta and manure pose residual antibiotics and antibiotic resistance genes (ARG’s) which are consequently passed to humans. This research is focused on developing and validating a solid phase extraction (SPE) procedure and an LC-MS/MS method for quantifying tylosin in cattle waste containing tylsoin. Tylosin is extracted from cattle waste samples by adding a sodium-EDTA buffer solution and methanol. The samples are cleaned up using Strata polymeric weak cation cartridges. Chemical analysis of the extracted tylosin is performed using a Varian 212-LC HPLC and Agilent 500 Ion Trap mass spectrometer detector. The results of this study will be presented which include the recovery percentage of tylosin in the cattle waste of the batch(cattle) which received tylosin in the form of feed and compared with the control group (cattle) which did not receive the tylsoin. Future work will include validation of the developed method to quantify tylosin in the cattle waste samples. Also, resistant genes in the cattle waste samples will be studied.