|GREEN, JAMES - University Of Hawaii|
|AL RWAHNIH, M - University Of California, Davis|
|OLMEDO, VELARDE - University Of Hawaii|
|MELZER, MICHAEL - University Of Hawaii|
|HAMIN, I - University Of Hawaii|
|BORTH, WAYNE - University Of Hawaii|
|Matsumoto Brower, Tracie|
|HU, JOHN - University Of Hawaii|
Submitted to: Tropical Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/9/2019
Publication Date: 2/5/2020
Citation: Green, J.C., Al Rwahnih, M., Olmedo, V.A., Melzer, M.J., Hamin, I., Borth, W.B., Matsumoto Brower, T.K., Wall, M.M., Hu, J.S. 2020. Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing. Tropical Plant Pathology. 45:64-72. https://doi.org/10.1007/s40858-019-00330-y.
Interpretive Summary: Pineapple mealybug wilt-associated virus (PMWaV) is an insect-vectored disease of pineapple, affecting all parts of the plant. There are three recognized PMWaV species and two putative species. In this paper, we describe the further genomic characterization of several PMWaV members of the genus Ampelovirus, family Closteroviridae using high-throughput sequencing (HTS). Resulted showed that PMWaV-4 is a distinct strain of PMWaV-1, but not a distinct Ampelovirus species. Therefore, we suggest that PMWaV-4 be designated PMWaV-1 strain 4. The genomic characterization of PMWaV species and strains is useful for understanding the complex etiology of mealybug wilt of pineapple and distinguishing among infectious and non-infectious virus subgroups.
Technical Abstract: In this study, we describe the further genomic characterization of several pineapple mealybug wilt-associated virus (PMWaV) members of the genus Ampelovirus, family Closteroviridae using high-throughput sequencing (HTS). PMWaV-2 is a necessary factor in the complex etiology of mealybug wilt disease of pineapple (MWP). The complete PMWaV-1 genome was described previously, but PMWaV-2, PMWaV-3, and the putative PMWaV-4 and PMWaV-5 genomes lack complete characterization. To further study MWP etiology through genomic characterization of PMWaVs, a cDNA library from total RNA of pineapple plants was generated and underwent HTS. A 13,150-nucleotide PMWaV-4 genome was assembled from the HTS data. This PMWaV-4 genome shared high amino acid (aa) homology to PMWaV-1. The RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homolog (HSP70) and coat protein (CP) genes of PMWaV-4 shared 88, 87, and 85% amino acid identity, respectively, to those of PMWaV-1 (AF414119) indicating PMWaV-4 is a distinct strain of PMWaV-1 but not a distinct ampelovirus species. Therefore, we suggest that PMWaV-4 be designated PMWaV-1 strain 4 (MH704743). Further, we used HTS to supplement the existing available sequences of the genomes PMWaV-2 (MH704741) and PMWaV-3 (MH704742) in their 5’-terminal regions.