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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #358600

Research Project: Molecular Genetic Tools Advancing the Application of Biotechnology for Crop Improvement

Location: Crop Improvement and Genetics Research

Title: RMCE methods of precise plant genome modification

item Thomson, James - Jim

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/26/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: This presentation discusses the research needed to develop the Recombinase Mediated Cassette Exchange (RMCE) technology as a tool for genome manipulation. Explanations are provided as to how genetic engineering of plants with novel traits can be applied. The definition of a “GAANTRY” and how this project has been developed to facilitate building of large complicated T-DNAs will be addressed. A schematic representation will be provided to demonstrate how this novel system can be used to ‘Stack’ genes and why this is necessary for complication genetic engineering practices. Finally, the concept of modularity and how the system can be adapted over time to newer genetic engineering techniques.