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Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Resequencing of bottle gourd germplasm and using QTL-seq to fine-map PRSV-W resistance in Bottle gourd (Lagenaria sinceraria)

item Chanda, Bidisha
item Ling, Kai-Shu
item WU, SHAN - Boyce Thompson Institute
item FEI, ZHANGJUN - Boyce Thompson Institute

Submitted to: Cucurbitaceae Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/13/2018
Publication Date: 11/12/2018
Citation: Chanda, B., Wu, S., Fei, Z., Ling, K. 2018. Resequencing of bottle gourd germplasm and using QTL-seq to fine-map PRSV-W resistance in Bottle gourd (Lagenaria sinceraria). Cucurbitaceae 2018 Conference Abstracts P49.

Interpretive Summary:

Technical Abstract: Bottle Gourd, a native of Africa is widely grown in the East Asian countries for food, medicine and decorative purposes. It is also used as an important rootstock to improve cold tolerance and disease resistance in other cucurbits, like watermelon and melon. Amongst other diseases, viral diseases are considered a major threat to cucurbit crop productions worldwide. The most prevalent viruses are aphid-transmitted papaya ring-spot virus watermelon strain (PRSV-W) and zucchini yellow mosaic virus (ZYMV). Commercially available bottle gourd cultivars are susceptible to these viral diseases. Traditional breeding takes years to develop a disease-resistant cultivar. With the recent advancement in the sequencing technologies, we aim to accelerate the molecular breeding for trait improvement. We recently sequenced the bottle gourd genome and used Genotyping-by-Sequencing to map the PRSV-W resistance locus in an F2 population. A dominant monogenic locus Prs was mapped in a 317.8 Kb region on the chromosome 1 still containing 39 annotated genes. In the current study, through QTLseq analysis on pooled libraries with 10 most PRSV resistant or susceptible individuals from six F3 populations, we aim to fine-map the Prs locus and to identify the candidate resistance gene for PRSV-W. In addition, through screening the USDA collections of bottle gourd germplasm, significant variations in the level of resistance to PRSV-W were identified in 154 Plant Introductions (PI) evaluated. Through genome re-sequencing (GBS) of these PI lines, we intend to use genome-wide association studies to confirm genes or SNPs linking to the PRSV-W resistance in bottle gourd.