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ARS Home » Plains Area » Stillwater, Oklahoma » Wheat, Peanut, and Other Field Crops Research » Research » Publications at this Location » Publication #357659

Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

Location: Wheat, Peanut, and Other Field Crops Research

Title: Characterization of Pm63, a new powdery mildew resistance gene identified in Iranian landrace PI 628024

Author
item Tan, Chengcheng
item Li, Genqiao
item Cowger, Christina
item CARVER, BRETT - Oklahoma State University
item Xu, Xiangyang

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/7/2018
Publication Date: 4/1/2019
Citation: Tan, C., Li, G., Cowger, C., Carver, B.F, Xu, X. 2019. Characterization of Pm63, a new powdery mildew resistance gene identified in Iranian landrace PI 628024. Journal of Theoretical and Applied Genetics. 132(4):1137-1144. https://doi.org/10.1007/s00122-018-3265-5.
DOI: https://doi.org/10.1007/s00122-018-3265-5

Interpretive Summary: Powdery mildew is globally important wheat disease causing severe yield losses, and plant host resistance is a preferred strategy to managing this disease. PI 628024 is an Iranian landrace exhibiting a wide spectrum of resistance to Blumeria graminis f. sp. tritici (Bgt) isolates collected from different regions of the USA. An F2 population and F2:3 lines derived from a cross PI 628024 x CItr 11349 were used in this study, and genetic analysis indicated that a single dominate gene, designated Pm628024, conferred resistance to Bgt isolate OKS(14)-B-3-1. Linkage analysis located Pm628024 to an interval of about 13.1 Mb on the long arm of chromosome 2B, spanning 710.3 – 723.4 Mb of the Chinese Spring reference sequence. Pm628024 is a new powdery mildew resistance gene which differs from other genes on the long arm of chromosome 2B in origins, genomic locations, and responses to a set of 16 US Bgt isolates. Pm628024 can be widely used to enhance powdery mildew resistance in the Great Plains, western, and southeastern regions of the USA.

Technical Abstract: Powdery mildew is a globally important wheat disease causing severe yield losses, and plant host resistance is a preferred strategy for managing this disease. The objective of this study was to characterize a powdery mildew resistance gene in Iranian landrace PI 628024, which exhibited a wide spectrum of resistance to representative Blumeria graminis f. sp. tritici (Bgt) isolates collected from different regions of the USA. An F2 population and F2:3 lines derived from the cross PI 628024 x CItr 11349 were used in this study, and genetic analysis indicated that a single dominant gene, designated Pm628024, conferred resistance to Bgt isolate OKS(14)-B-3-1. Linkage analysis located Pm628024 to an interval of about 13.1 Mb on the long arm of chromosome 2B, spanning 710.3 – 723.4 Mb of the Chinese Spring reference sequence. Bin mapping assigned Pm628024 to the terminal bin 2BL6-0.89-1.0, with a genetic distance of 1.1 cM proximal to STS marker BCD135-2, and 0.6 cm distal to SSR marker STARS419. Allelism tests indicated that Pm628024 is a new powdery mildew resistance gene which differs from other genes in the terminal bin in origins, genomic locations, and responses to a set of 16 representative US Bgt isolates. Pm628024 can be widely used to enhance powdery mildew resistance in the Great Plains, western, and southeastern regions of the USA.