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Title: Invivo generation of dihaploids in tall fescue

item Kindiger, Bryan

Submitted to: Molecular Breeding of Forage and Turf Conference
Publication Type: Abstract Only
Publication Acceptance Date: 9/18/2018
Publication Date: 3/25/2019
Citation: Kindiger, B.K. 2019. Invivo generation of dihaploids in tall fescue [abstract]. Molecular Breeding of Forage and Turf Conference. Available at:

Interpretive Summary: Abstract only

Technical Abstract: Within the Lolium-Festuca genome complex there is a need for novel breeding approaches that can facilitate the rapid development of improved germplasm, cultivars or material for molecular studies. A novel diploid, Lolium multiflorum (Lolium perenne L. subsp. multiflorum (Lam.) Husnot (syn. Lolium multiflorum Lam.) line has been identified that induces genome loss and dihaploid generation following hybridization with hexaploid, L. arundinaceum (Schreb.) Darbysh.) (syn.Festuca arundinacea Schreb.). Lolium x Festuca hybridizations are not a new area of breeding research; however, the generation of dihaploid products from Lolium x Festuca hybridizations is novel. The generation of homozygous, dihaploid lines of tall fescue creates numerous opportunities for enhanced breeding methods and molecular research in this species. This research describes the utilization of novel L. multiflorum inducer lines to produce dihaploid L. arundinaceum. The generation of dihaploids is structured around a mitotic, genome loss character that is conferred by the novel inducer lines.