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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #354781

Research Project: Characterization of Colonization of Shiga Toxin-producing Escherichia coli (STEC) in Cattle and Strategies for Effective Preharvest Control

Location: Food Safety and Enteric Pathogens Research

Title: Fecal microbiota changes associated with dehorning and castration stress primarily affects light-weight dairy calves

Author
item MIR, RAIES - Orise Fellow
item KLEINHENZ, MICHAEL - Iowa State University
item COETZEE, JOHANN - Kansas State University
item Allen, Heather
item Kudva, Indira

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/18/2018
Publication Date: 1/23/2019
Citation: Mir, R.A., Kleinhenz, M.D., Coetzee, J.F., Allen, H.K., Kudva, I.T. 2019. Fecal microbiota changes associated with dehorning and castration stress primarily affects light-weight dairy calves. PLoS One. 14(1):e0210203. https://doi.org/10.1371/journal.pone.0210203.
DOI: https://doi.org/10.1371/journal.pone.0210203

Interpretive Summary: Stress can lead to changes in normal behavior, immunity, metabolism and growth, which in turn can negatively affect an animal's health. Gastrointestinal tract (GIT) of cattle contains number of different types of bacteria, collectively called as microbiota. Disturbances in GIT microbiota can disrupt the digestive system and hence, an animal’s metabolisma and overall health. This study evaluated the biological effects of dehorning and castration stress on GIT microbiota of calves. We observed that stress causes changes in GIT microbiota; a decrease in microbial diversity was especially seen in light-weight calves after dehorning or castration. In order to reduce this and other adverse effects of stress in cattle, further studies are needed to establish this causal association between stress and GIT microbiota changes and determine how this disturbance maybe definitively identified and remedied.

Technical Abstract: Gastrointestinal tract (GIT) microbiota and stress can impact animal health. Studies have shown that perturbations in the GIT microbiota can influence host health and productivity by affecting physiological homeostasis, metabolism, hematopoiesis and inflammation. The present study aimed to evaluate possible effects of dehorning and castration stress on the GIT microbiota of dairy calves. Dehorning and castration are routinely performed on over 90 percent of dairy farms, and analgesics like flunixin meglumine (FLU) are given at the time of these procedures to reduce pain. We analyzed fecal microbiota of 24 weaned male dairy calves at two different stages in their life (at 10 weeks for dehorning and 36 weeks age for castration) to determine any GIT microbiota changes due to these stressful procedures and the FLU treatment. Dehorning was performed using an electrocautery dehorner applied to the horn for 10 seconds, and surgical castration was used as the castration method. Our analysis showed that the Shannon diversity index was significantly higher in animals that were not dehorned compared to dehorned animals. Castration stress also resulted in a significant decrease in Shannon diversity index, which was more pronounced in lower weight calves. Body weight and stress had significant effects on the taxonomic profiles of the GIT microbiota. There was a significant difference in the GIT bacterial community structure between heavy- and light-weight calves at Day 3 after castration but not at Day 0 (prior to castration). Our results indicate that dehorning and castration stress reduced microbial diversity of the GIT microbiota, but only in light-weight calves. This work is important for elucidating biological effects of stress on dairy calves and identifying potential modulation points in the microbiota of these food-producing animals to improve animal health and production.